Chitosan preparation and application thereof on controlling black spot of harvested sweet potato
A technology of chitosan and black spot disease, which is applied in the application field of chitosan in the prevention and treatment of sweet potato black spot disease, can solve the problems of environmental pollution and human harm, and achieve the prevention and treatment of black spot disease with low cost and good control effect Effect
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[0016] The specific technical scheme is as follows:
[0017] 1. The preparation method of chitosan preparation
[0018] Weigh 1.5g of chitosan powder with a viscosity of 60-70mPa.s and a degree of deacetylation of 85-90%, irradiate it with an ultraviolet lamp for 12h in an ultra-clean workbench, add an appropriate amount of sterile water, and stir slightly until the chitosan powder is in the Fully swell in water, add 0.5 mL of acetic acid dropwise, stir magnetically until the chitosan is completely dissolved, add sterile water to make up to 100 mL, and prepare a chitosan mother solution with a concentration of 15 mg / mL. The concentration of the chitosan mother liquor can be adjusted to 0.25-2mg / mL according to the doubling dilution method, and the pH can be adjusted to 6.0-6.5 with NaOH solution. When used, the concentration of chitosan can be adjusted as needed.
[0019] 2. Effect of chitosan preparation on mycelial growth and sporulation of sweet potato black spot fungus ...
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