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A silicon-based nanobowl array chip and its preparation method and application

An array chip and nano bowl technology, which is applied in the field of silicon fund nano bowl array chip and its preparation, can solve the problem that the application field needs to be expanded, and achieve the effects of improving reproducibility and stability, good reproducibility and easy operation.

Active Publication Date: 2019-12-10
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current application of nanobowl arrays is mainly based on laboratory research work, and its application fields have yet to be expanded.

Method used

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  • A silicon-based nanobowl array chip and its preparation method and application
  • A silicon-based nanobowl array chip and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1, the preparation of silicon foundation nanobowl array chip

[0047] Such as figure 1 As shown, for the fabrication of the silicon-based nanobowl array chip, firstly, the single-layer colloidal crystal floating at the gas-liquid interface is used as a template, and the ordered gold nanobowl array is prepared by reducing gas diffusion and chemical deposition reaction, and the prepared The gold nanobowl array chip was fished on the 4-mercaptopropyltrimethoxysilane-modified silicon chip, and Au-S bonds were formed between them to obtain the silicon-based nanobowl array chip, which was modified and improved as a MALDI - MS target board. Mix the analyte and the matrix in a certain ratio, take a microliter volume of the mixed sample solution, drop it on the silicon-based nanobowl array chip, and control the drying temperature and relative humidity of the mixed droplet to obtain a uniform crystallization of the "sample-matrix" . The silicon-based nanobowl array...

Embodiment 2

[0050] Embodiment 2, the preparation of silicon foundation nanobowl array chip

[0051] First select polystyrene microspheres with good monodispersity and uniform size (the diameter of the microspheres can be selected from 300nm to 600nm), and a single-layer colloidal crystal film of polystyrene microspheres obtained by self-assembly at the gas-liquid interface is used as the preparation method. The template of the nanobowl array is transferred to the surface of 2mM chloroauric acid solution, and then the mixed solution of 7mL hydrazine hydrate and 3mL concentrated ammonia water is used as the original solution of reducing gas diffusion, and reacted in a desiccator for 2h to obtain a certain mechanical strength. The ordered gold nanobowl array (composite polystyrene microsphere template, the inner diameter of the gold nanobowl array is 200nm-450nm). The clean silicon chip that will be used for spreading gold nanobowl array is done hydrophilic treatment in advance, and hydrophi...

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Abstract

The invention discloses a silicon-based gold nano bowl array chip, and a preparation method and an application thereof. The silicon-based gold nano bowl array chip comprises a gold nano bowl array and a sulfhydryl modified silicon slice; the gold nano bowl array is spread on the sulfhydryl modified silicon slice, and Au-S bond connection is formed between the gold nano bowl array and the sulfhydryl modified silicon slice. The silicon-based gold nano bowl array chip is applied in MALDI-MS detection of biomolecules. The method for MALDI-MS detection of the biomolecules comprises the following steps: dropwise adding a mixed liquid of a biomolecule sample and a matrix on the silicon-based gold nano bowl array chip, drying and crystallizing, attaching the treated silicon-based gold nano bowl array chip onto an MALDI-MS target plate, and then carrying out MALDI-MS detection. The silicon-based gold nano bowl array chip has the advantages of high sensitivity, good reproducibility, simple operation and low cost.

Description

technical field [0001] The invention relates to a silicon-funded nanobowl array chip and a preparation method and application thereof, belonging to the field of biotechnology. Background technique [0002] Matrix-assisted laser desorption / ionization mass spectrometry (MALDI-MS) is a soft ionization mass spectrometry detection method, which introduces an organic acid that can absorb incident ultraviolet laser energy as a matrix, completely desorbs and ionizes the sample, and solves the problem of biological samples in the traditional The laser desorption / ionization mass spectrometer (LDI-MS) is easy to produce fragment ions in the detection, which provides a suitable method for the analysis of thermally unstable, polar, and difficult to gasify biological macromolecules, fundamentally It solves the problem that LDI-MS cannot detect biological macromolecules. The reason why MALDI-MS can be widely used in the field of biochemical detection is inseparable from its advantages. F...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62
CPCG01N27/628G01N33/6851
Inventor 林金明李玮玮
Owner TSINGHUA UNIV
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