Three-dimensional cell cultivator based on temperature-responsive liquid marbles and construction and application methods of three-dimensional cell cultivator
A temperature-responsive, three-dimensional cell technology, used in tissue cell/virus culture devices, biochemical equipment and methods, and methods for supporting/immobilizing microorganisms. The overall microenvironment required for growth and differentiation, the lack of three-dimensional scaffolds, etc., achieve the effects of excellent stability and gas permeability, easy mass production, and broad application prospects.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0032] (1) Preparation of hybrid particles
[0033] When the inorganic micro-nanoparticles are nano-silica, and the temperature-sensitive polymer is poly-N-isopropylacrylamide PNIPAM homopolymer, use 3-aminopropyltriethoxysilane and 2-bromoisobutyryl bromide with SiO 2 reaction to get SiO 2 -Br initiator, and then use surface-initiated atom transfer radical polymerization to connect PNIPAm to SiO 2 surface, to obtain SiO with a surface grafting rate of 40% 2 @PNIPAm.
[0034] (2) Construction of liquid marble three-dimensional cell culture device
[0035]With Hep G2 (approximately 10 per 100 μL 4 ) The cell culture medium is (DMEM medium (Gibco), 10% calf serum, and penicillin solution 100U, NaHCO 3 buffer solution) was used as a cell culture medium and temporarily stored at 5°C. Spread the synthesized temperature-sensitive hybrid particles on the plastic substrate, add 150 μL of cell culture solution dropwise with a micro-injector, and then use the micro-injector to dr...
example 2
[0039] (1) Preparation of hybrid particles
[0040] When the inorganic micro-nanoparticles are nano-silica, and the temperature-sensitive polymer is PNIPAm / PAA, a copolymer of poly-N-isopropylacrylamide and polyacrylic acid, 3-aminopropyltriethoxysilane and 2- Bromoisobutyryl bromide and SiO 2 reaction to get SiO 2 -Br initiator, and then graft PNIPAm and PAA monomers to SiO by initiating atom transfer radical polymerization on the graft surface 2 surface, to obtain a surface grafting rate of 40% of SiO 2 @PNIPAM / PAA particles.
[0041] (2) Construction of liquid marble three-dimensional cell culture device
[0042] With Hep G2 (approximately 10 per 100 μL 4 ) The cell culture medium is (DMEM medium (Gibco), 10% calf serum, and penicillin solution 100U, NaHCO 3 buffer solution) was used as a cell culture medium and temporarily stored at 5°C. Spread the synthesized temperature-sensitive hybrid particles on the plastic substrate, add 150 μL of cell culture solution dropwi...
example 3
[0046] (1) Preparation of hybrid particles
[0047] When the temperature-sensitive polymer is nano-ferric oxide, the silane coupling agent 2-(4-chlorosulfonylphenyl) ethyl trichlorosilane (CTCS) is grafted onto Fe 3 o 4 surface, and then the PNIPAM / PEG copolymer was grafted onto Fe by surface-initiated atom transfer radical polymerization 3 o 4 surface, to obtain a surface grafting rate of 30% Fe 3 o 4 @PNIPAm / PAA Particles.
[0048] (2) Construction of liquid marble three-dimensional cell culture device
[0049] With Hep G2 (approximately 10 per 100 μL 4 ) The cell culture medium is (DMEM medium (Gibco), 10% calf serum, and penicillin solution 100U, NaHCO 3 buffer solution) was used as a cell culture medium and temporarily stored at 5°C. Spread the synthesized temperature-sensitive hybrid particles on the plastic substrate, add 120 μL of cell culture solution dropwise with a micro-injector, and then use the micro-injector to drive the droplet to roll, so that the surf...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com