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Method for extracting polysaccharide from lycium barbarum

A technology of wolfberry fruit and polysaccharide, which is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc.

Inactive Publication Date: 2018-01-05
贵州中科健生物医药有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the role of Lycium barbarum polysaccharide in the treatment of Alzheimer's disease.

Method used

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  • Method for extracting polysaccharide from lycium barbarum
  • Method for extracting polysaccharide from lycium barbarum
  • Method for extracting polysaccharide from lycium barbarum

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Embodiment

[0024] A method for extracting polysaccharides from wolfberry, comprising the steps of:

[0025] (1) Polysaccharide extraction: 1000 g of dried Lycium barbarum fruit was pulverized by a pulverizer, and 20 L of deionized water was added. At 55° C., 30 g of cellulase, 10 g of amylase, and 5 g of papain were added for extraction for 1 h, and then Raise the temperature to 100°C to inactivate the enzyme, use a GM-21 centrifuge to centrifuge at 8000rpm / min for 15min, discard the precipitate, concentrate the filtrate to a small volume, and then dialyze against flowing water with a dialysis bag with a molecular cutoff of 3500Da After 3 days, heat and concentrate the dialyzed inner solution to 3L, centrifuge to discard the precipitate, add 15L of 95% ethanol to the supernatant under stirring, let it stand overnight, and centrifuge to obtain a precipitate, which is washed alternately with absolute ethanol and acetone 3 times, centrifuged, and the precipitate was vacuum-dried at 50°C to ...

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Abstract

The invention relates to polysaccharide extracted from lycium barbarum, an extraction method of the polysaccharide and the application of the polysaccharide in preparation of a drug for treating an Alzheimer's disease and specifically relates to a method for extracting polysaccharide LBP1C-2 (lycium barbarum polysaccharide 1C-2) from the lycium barbarum. The method comprises the following steps: extracting enzyme-water combined extraction lycium barbarum crude polysaccharide from the lycium barbarum and purifying the crude polysaccharide, so as to obtain the polysaccharide LBP1C-2; determiningthe polysaccharide LBP1C-2 as pectin through acid hydrolysis, methylation and nuclear magnetic resonance analysis. An in-vitro experiment shows that the polysaccharide LBP1C-2 can inhibit the generation of CHO / APPBACE1 cells of stable transfection APP and BACE1 and A beta 42 in HEK293-APPsw cells formed by mutation of stable transfection APP Switish in a dose-dependent manner, and the pectin caninhibit the aggregation of A beta 42.

Description

technical field [0001] The invention relates to the field of polysaccharide extraction, in particular to a method for extracting polysaccharides from wolfberry. Background technique [0002] Alzheimer's disease, also known as Alzheimer's disease, is a progressive neurodegenerative disease. The main clinical manifestations are gradual memory loss, cognitive dysfunction, abnormal behavior and social impairment. With the increasing aging of the population, the incidence of Alzheimer's disease is also increasing year by year, and it has become one of the most important health issues of public concern. The deposition and abnormal expression of β-amyloid protein in the brain is currently considered to be the core link of Alzheimer's disease. Taking β-amyloid protein as the target, it can reduce β-amyloid from the source by interfering with APP metabolism. The production of protein is currently a research hotspot in the treatment of Alzheimer's disease. [0003] Lycium barbarum b...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P25/28
Inventor 丁侃周立爽
Owner 贵州中科健生物医药有限公司
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