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Cottonseed protein polypeptide with high angiotensin converting enzyme (ACE) inhibition activity and preparation method of cottonseed protein polypeptide

A cottonseed protein and inhibitory activity technology, which is applied in the field of cottonseed protein polypeptide with high ACE inhibitory activity and the preparation thereof, to achieve the effect of improving blood pressure lowering activity

Inactive Publication Date: 2018-01-09
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of Plastein reaction to improve the antioxidant activity of ACE inhibitory peptide derived from cottonseed protein

Method used

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  • Cottonseed protein polypeptide with high angiotensin converting enzyme (ACE) inhibition activity and preparation method of cottonseed protein polypeptide
  • Cottonseed protein polypeptide with high angiotensin converting enzyme (ACE) inhibition activity and preparation method of cottonseed protein polypeptide
  • Cottonseed protein polypeptide with high angiotensin converting enzyme (ACE) inhibition activity and preparation method of cottonseed protein polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Reagents and materials

[0026]

[0027]

[0028] 2. Instruments and equipment

[0029] Evolution RC High Speed ​​Freezer

Kejun Corporation

PB-10 precision pH meter

Sartorius

UV2000 UV-Vis Spectrophotometer

Longnike Instrument Co., Ltd.

JJ-2 tissue masher

Jiangsu Jintan Huanyu Scientific Instrument Factory

WS28 electric constant temperature water bath

Xi'an Hepu Biotechnology Co., Ltd.

TCL-16M High Speed ​​Desktop Refrigerated Centrifuge

Hunan Xiangyi Centrifuge Instrument Co., Ltd.

Upright Desktop Freeze Dryer

thermoelectric corp. of america

HOI-IB digital constant temperature magnetic stirrer

Shanghai Meiyingpu Instrument Manufacturing Co., Ltd.

[0030] 3. Experimental method

[0031] 3.1 Extraction of cottonseed protein

[0032] Add distilled water to the dephenolized cottonseed meal powder according to the ratio of material to liquid 1:25 (g / mL), adjust the pH v...

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PUM

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Abstract

The invention aims at providing a method for improving the angiotensin converting enzyme (ACE) inhibition activity of cottonseed protein ACE inhibition peptide. The ACE inhibition peptide is preparedby taking cottonseed protein as a raw material and carrying out plastein reaction modification on the cottonseed protein ACE inhibition peptide; the ACE inhibition rate of the prepared product reaches90% or above, so that the activity of the cottonseed protein ACE inhibition peptide is improved; therefore, a new way is provided for the preparation of the natural efficient ACE inhibitor, and a basis is provided for the comprehensive development and utilization of cottonseed resource.

Description

technical field [0001] The invention relates to a cottonseed protein ACE inhibitory peptide and a preparation method thereof, in particular to a cottonseed protein polypeptide with high ACE inhibitory activity and a preparation method thereof. Background technique [0002] Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptide carboxypeptidase (Dippeptidely Carbononxypeptidase) widely present in human tissues and plasma, and its relative molecular weight is 120,000-150,000. It mainly exists in vascular endothelial cells, and is most abundant in pulmonary capillary cells. ACE has the effect of raising blood pressure, and inhibiting the activity of ACE is beneficial to the treatment of hypertension. [0003] Studies have found that many natural proteolytic peptides have ACE inhibitory activity, known as ACE inhibitory peptides. These bioactive peptides have great potential in the prevention and treatment of hypertension. Due to the special advantages of food-der...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/30
Inventor 高丹丹陈士恩田晓静李明生丁功涛
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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