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Glioma diagnostic marker circ9:135881633|135883078 and its application

A technology for glioma and exosomes, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems that the survival rate is not optimistic and the survival rate of glioma patients has not been significantly improved, and achieves a good and rich Integrating effect, improving clinical treatment effect, convenient and accurate diagnosis

Active Publication Date: 2020-09-11
XIANGYA HOSPITAL CENT SOUTH UNIV
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AI Technical Summary

Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

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  • Glioma diagnostic marker circ9:135881633|135883078 and its application
  • Glioma diagnostic marker circ9:135881633|135883078 and its application
  • Glioma diagnostic marker circ9:135881633|135883078 and its application

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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] 1. One glioma cell line is U251 cell line, and five primary glioma cell lines are 1216, 1125, 1124B, 1124C, and 0128C, all provided by the Institute of Cancer, School of Basic Medicine, Central South University.

[0022] 2. The serum samples of 40 patients with glioma were provided by Xiangya Hospital, and the serum samples of 15 normal patients were healthy individuals who underwent community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0023] 2. Research Methods

[0024] 1. Extraction of RNA from cells, exosomes secreted by cells, and glioma / normal serum exosomes

[0025] A. Extraction of cellular RNA

[0026] After the above six kinds of cells grow to an appropriate density, ...

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Abstract

The invention belongs to the field of biological technology, and discloses a glioma diagnostic marker circ9:135881633 / 135883078, and applications thereof. According to study, it is found for the firsttime that exosome secreted by glioma possesses obvious enrichment effect on circ9:135881633 / 135883078; at the same time, it is found that the expression level of circ9:135881633 / 135883078 in serum exosome of patients with glioma is obviously higher than that of a normal reference group (P=0.0142), it is shown by ROC analysis that circ9:135881633 / 135883078 possesses relatively high diagnosis valueon glioma (AUC=0.953, P<0.001, sensitivity is 83.3%, and specificity is 96.7%). Early rapid noninvasive diagnosis on patients with glioma can be realized via measuring expression level of circ9:135881633 / 135883078 in serum exosome of patients with glioma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker circ9:135881633|135883078 for glioma diagnosis, and the application of a reagent for detecting the marker to prepare a glioma diagnostic preparation, and a kit. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based on clinical,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
Inventor 李沛瑶武明花孙英男冯紫阳刘强陈琼余志斌
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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