Application of fulvestrant in preparation of drugs for treating nonfunctional pituitary adenoma
A technique for fulvestrant, pituitary adenoma, applied in the fields of biochemistry and medicine
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[0023] 1.1 Specimen source
[0024] Specimens were obtained from surgically resected tumor tissues of patients with pituitary adenomas. Preoperative diagnosis was made based on clinical manifestations, serum hormone levels and imaging examinations, and then confirmed according to intraoperative findings, postoperative pathology and immunohistochemical staining. It was non-functioning pituitary adenoma: empty cell adenoma, macrophage Oncocytoma, gonadotroph adenoma, 30 cases of each non-functioning pituitary adenoma.
[0025] 1.2 Main reagents
[0026] Trypsin, DMEM medium, fetal bovine serum, dimethyl sulfoxide, and polylysine are all commercially available products.
[0027] 1.3 Cell culture
[0028] The surgically removed pituitary adenoma tissue is put into serum-free DMEM culture medium under aseptic operation, rinsed with sterile PBS in the ultra-clean bench for 2-3 times, removes connective tissue, necrotic tissue and blood clots, and cuts the specimen with ophthalmic...
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