Method for increasing fermentation yield of gentamicin B

Abstract

The invention provides a method for increasing the fermentation yield of gentamicin B. The method comprises the following steps: inoculating a strain cultured on a seed culture medium into a fermentation culture medium of a fermentation tank, and fermenting at 33-37 DEG C, wherein the tank pressure is 0.03-0.05MPa, and the ventilation rate is (1:0.5)-(1:1.5)vvm; and replenishing an inorganic saltsolution when fermentation is carried out for 20-30 hours, replenishing an inorganic salt solution again when fermentation is carried out for 40-50 hours, performing material replenishing culture whenfermentation is carried out for 60-70 hours, maintaining the dissolved oxygen concentration inside the fermentation tank to be greater than a set value by changing rotation speeds, and performing column analysis and concentration freeze-drying on fermentation broth, thereby obtaining gentamicin B. By adopting the method, culture is carried out by using a method that materials are replenished andfermentation is carried out in different batches, the inorganic salt solution is put into the fermentation culture medium at different time periods of fermentation, and the yield of gentamicin B is increased; and by adopting the method, the yield of gentamicin B is increased by 80%-90%, the fermentation period is shortened, and the production cost of gentamicin B prepared by using a fermentation method is reduced.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for increasing the fermentation yield of gentamicin B. Background technique [0002] Gentamicin is an aminoglycoside antibiotic mainly used to treat bacterial infections, especially those caused by Gram-negative bacteria. Gentamicin can bind to the 30s subunit of bacterial ribosomes and block bacterial protein synthesis. Gentamicin [Gentamicin, gentamycin (gentamycin)] is extracted from the fermentation broth of Actinomycetes Monospore. It is an alkaline compound and is a commonly used aminoglycoside antibiotic. [0003] Gentamicin B is fermented from Micromonas rubrum. The yield of gentamicin B in the wild-type strain was very low. At present, the strains of gentamicin B are mainly obtained through strain mutation screening. Gentamicin B can only be produced artificially by fermentation. However, in the process of preparing gentamicin B by fermentation, ...

AI Technical Summary

Problems solved by technology

Gentamicin B can only be produced artificially by fermentation

However, in the process of preparing gentamicin B by fermentation, it is usually extracted from the by-product of gentamicin, so there are many components, low content, large separation difficulty and low purity.

Simultaneously, the technology for preparing gentamicin B by traditional fermentation method also has the defects of long fermentation period and high preparation cost of fermentation method

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