Method for producing oxytetracycline calcium premix by streptomyces rimosus fermentation

A technology of Streptomyces fissures and oxytetracycline calcium, which is applied in the field of veterinary medicine, can solve problems such as high cost, high energy consumption, and excessive dust, and achieve the effects of less heat source substances, pollution prevention, and stable metabolism

Inactive Publication Date: 2018-01-12
金河生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production methods of oxytetracycline calcium premix are mainly some traditional methods such as spray drying method and high temperature drying method, but these methods have disadvantages such as high energy consumption, high cost, much dust, and not being environmentally friendly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Production of oxytetracycline calcium premix by fermentation of Streptomyces fissures

[0026] (1) Seed preparation

[0027] Streptomyces chamotis spores were inoculated onto the mother slant containing the spore medium, and cultivated for 68 hours at a temperature of 34° C. under a condition of 45% humidity; Cultivate for 22 hours at 30° C. and 45% humidity until the spores mature.

[0028] The spore culture medium is composed of the following components in terms of mass percentage concentration: bran 5.5%, MgSO 4 0.004%, KH 2 PO 4 0.01%, (NH 4 ) 2 HPO 4 0.015%, agar powder 2.0%, and the balance is water.

[0029] (2) Seed tank cultivation

[0030] Transfer the mature spores to seed tanks for cultivation, firstly carry out primary seed tank cultivation, the cultivation conditions are: cultivation temperature 32°C, ventilation rate 1:2.0v / v / min, tank pressure 0.02MPa, stirring speed 210rpm, cultivation time 25 hours; then, according to the ratio of the volume...

Embodiment 2

[0043] Production of oxytetracycline calcium premix by fermentation of Streptomyces fissures

[0044] (1) Seed preparation

[0045] Streptomyces schizophrenia spores were inoculated onto the mother slant containing the spore medium, and the temperature was 36° C., and the humidity was 50% and cultivated for 69 hours; Cultivate for 22 hours at 28°C and 50% humidity until the spores mature.

[0046] The spore culture medium is composed of the following components in terms of mass percentage concentration: bran 5.5%, MgSO 4 0.004%, KH 2 PO 4 0.01%, (NH 4 ) 2 HPO 4 0.015%, agar powder 2.0%, and the balance is water.

[0047] (2) Seed tank cultivation

[0048] Transfer mature spores to seed tanks for cultivation, firstly carry out primary seed tank cultivation, the cultivation conditions are: cultivation temperature 32°C, ventilation rate 1:1.8v / v / min, tank pressure 0.02MPa, stirring speed 220rpm, cultivation time 32 hours; then, according to the ratio of the volume rati...

Embodiment 3

[0061] Production of oxytetracycline calcium premix by fermentation of Streptomyces fissures

[0062] (1) Seed preparation

[0063] Inoculate Streptomyces chamotis spores on the mother slant containing the spore medium, and cultivate it for 66 hours at a temperature of 35° C. under the condition of 42% humidity; Cultivate for 23 hours at 30° C. and 50% humidity until the spores mature.

[0064] The spore culture medium is composed of the following components in terms of mass percentage concentration: bran 5.5%, MgSO 4 0.004%, KH 2 PO 4 0.01%, (NH 4 ) 2 HPO 4 0.015%, agar powder 2.0%, and the balance is water.

[0065] (2) Seed tank cultivation

[0066] Transfer mature spores to seed tanks for cultivation, firstly carry out primary seed tank cultivation, the cultivation conditions are: cultivation temperature 31°C, ventilation rate 1:1.5v / v / min, tank pressure 0.02MPa, stirring speed 220rpm, cultivation time 35 hours; then, according to the ratio of the volume ratio o...

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PUM

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Abstract

The invention relates to a method for producing oxytetracycline calcium premix by streptomyces rimosus fermentation, and belongs to the technical field of veterinary drug. In the method, the technicalconditions of a seed preparation process are optimized, so that the prepared seed has the characteristics of stable metabolism, small batch difference, high hypha concentration, small protein content, less heat-source matters and the like. Meanwhile, according to the method, culture medium ingredients are optimized, the culture medium is used for culturing the seeds so as to be favorable for thesynthesis of an oxytetracycline special structure and the improvement of the yield of the oxytetracycline calcium premix and also be favorable for subsequently separating and purifying the oxytetracycline calcium premix. A high-pressure diaphragm sheet frame machine is adopted to filter, and a flash evaporation dryer is used for drying. Compared with the traditional production method for the oxytetracycline calcium premix, the method disclosed by the invention is characterized in that a fermentation unit and yield can be effectively improved, production cost is lowered, meanwhile, the use of chemical organic solvent is avoided, pollution caused by the organic solvent for the oxytetracycline is avoided, and harm on animals is avoided.

Description

technical field [0001] The invention belongs to the technical field of veterinary medicines, and in particular relates to a method for producing oxytetracycline calcium premix by fermenting Streptomyces chastis. Background technique [0002] Oxytetracycline is a broad-spectrum antibiotic, which has inhibitory effects on Gram-positive bacteria and Gram-negative bacteria, and also has certain inhibitory effects on Chlamydia, Rickettsia, Mycoplasma, Spirochetes, etc. Oxytetracycline is mainly used to prevent and treat E. coli infection in livestock and poultry, Salmonella infection (such as calf pullorum, lamb dysentery, piglet yellow and white diarrhea, young animal paratyphoid, etc.), Pasteurella infection, Brucella infection and swine panting disease, chicken Chronic respiratory diseases are also commonly used to treat rickettsial disease in dogs, infectious anemia in cats, prevent chlamydial disease and spirochetal disease in dogs, and be used locally for endometritis and t...

Claims

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Application Information

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IPC IPC(8): C12P29/00A23K20/195A23K20/24C12R1/59
Inventor 谢昌贤刘运添李兰枝王月清邓维康王鹏飞
Owner 金河生物科技股份有限公司
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