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Glioma diagnostic marker circ7:100812747|100813208 and application

A glioma and marker technology, applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problems of poor survival rate and no obvious improvement in survival rate of glioma patients , achieve good enrichment effect, improve clinical treatment effect, convenient and accurate diagnosis

Inactive Publication Date: 2018-01-16
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

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  • Glioma diagnostic marker circ7:100812747|100813208 and application
  • Glioma diagnostic marker circ7:100812747|100813208 and application
  • Glioma diagnostic marker circ7:100812747|100813208 and application

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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] 1. One glioma cell line is U251 cell line, and five primary glioma cell lines are 1216, 1124C, 1124B, 1104, and 0128C, all provided by the Institute of Cancer, School of Basic Medicine, Central South University.

[0022] 2. The serum samples of 40 patients with glioma were provided by Xiangya Hospital, and the serum samples of 15 normal patients were healthy individuals who underwent community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0023] 2. Research methods

[0024] 1. Extraction of RNA from cells, exosomes secreted by cells, and glioma / normal serum exosomes

[0025] A. Extraction of cellular RNA

[0026] After the above six kinds of cells grow to an appropriate density, ...

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Abstract

The invention belongs to the technical field of biology and discloses a glioma diagnostic marker circ7:100812747|100813208 and application. In the invention, exosomes secreted by glioma cells are found to have an obvious enrichment effect on the circ7:100812747|100813208 for the first time; meanwhile, the expression level of the circ7:100812747|100813208 in serum exosomes of glioma patients is found to be remarkably improved compared with that of a control group (p is equal to 0.0444); ROC (Receiver Operating Curve) analysis shows that the circ7:100812747|100813208 has higher diagnostic value(AUC is equal to 0.924, p is equal to 0.001, and the sensitivity and the specificity are 83.3 percent and 87.5 percent respectively) to glioma. Therefore, early rapid noninvasive diagnosis of the glioma patients can be realized by detecting the expression level of the circ7:100812747|100813208 in the serum exosomes of the glioma patients.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker circ7:100812747|100813208 for glioma diagnosis, and the application of a reagent for detecting the marker to prepare glioma diagnostic preparations, and a kit. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based on clinical, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
Inventor 武明花冯紫阳李沛瑶佘晓玲孙英男刘长红刘涛
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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