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Manufacturing method and application of immunoliposome compound nanoparticle biochip

A technology of immunoliposomes and biochips, applied in biological testing, instruments, measuring devices, etc., can solve the problem of lack of specificity, simultaneous detection and analysis of various biological samples and molecules, lack of rapid and simultaneous detection analysis and other issues

Active Publication Date: 2018-01-16
SPOT BIOSYST LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Traditional biomolecular chip analysis techniques usually lack the characteristics of fast and simultaneous detection and analysis of various biological samples and molecules. For cell samples and extracellular vesicle samples, because the sample surface usually has a variety of membrane proteins or other receptors As a result, biochips need to perform complex sample pretreatment procedures before analysis, and then use biochips for analysis to achieve reliable analysis results.
[0003] US Patent Publication No. US 20140094383 discloses a biochip. However, the biochip uses cationic nanomolecules to modify the surface of the biochip so that the biochip can have a better affinity with the negatively charged cell membrane due to electrostatic interaction, but The above-mentioned biochip modified by cationic nanomolecules does not solve the problem of being able to simultaneously detect and analyze various biological samples and molecules, and the biochip modified by cationic nanomolecules is also deficient in membrane proteins and extracellular vesicles on the surface of cell samples. Specificity, thus limiting the field of its use

Method used

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  • Manufacturing method and application of immunoliposome compound nanoparticle biochip
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  • Manufacturing method and application of immunoliposome compound nanoparticle biochip

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no. 1 example

[0032] According to the first embodiment of the present invention, a method for manufacturing a nanoparticle biochip and a method for in vitro detection and identification of membrane proteins, ribonucleic acid, and deoxyribonucleic acid in biological samples are provided, the steps of which include: providing a biological sample, the biological sample includes Membrane protein, ribonucleic acid and deoxyribonucleic acid; provide antibody biochip, the antibody biochip includes antibodies and molecular probes, the above-mentioned antibody biochip captures cells and extracellular vesicles with antibody specificity in vitro On the surface of the chip, the molecular probes include nanoparticles, fluorescently labeled antibodies, molecular beacons and aptamer beacons. The above-mentioned molecular probes are used to detect membrane proteins, ribonucleic acid and deoxyribonucleic acid in biological samples in vitro; Make the above-mentioned biological sample contact the antibody bioc...

specific Embodiment

[0033] In a specific embodiment, the above-mentioned cells and extracellular vesicles with antibody specificity are antigens with corresponding antibodies.

[0034] According to the second embodiment of the present invention, the manufacturing method of the immunoliposome complex nanoparticle biochip of the present invention includes the following steps: providing a substrate, which includes glass, silicon wafer, polymer and ceramics; A gold film is formed on the surface of the substrate, an interface layer is included between the gold film and the surface of the substrate, and the interface layer includes 3-thiol propyltrimethoxysilane; composition is provided, and the composition includes lipid An anchor molecule, a linker, the linker includes a biotin-linked thiol molecule with an oxirane unit and a lateral spacer molecule; adding an avidin, the avidin includes neutravidin and Streptavidin, the above-mentioned avidin and the above-mentioned components react on the surface o...

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Abstract

The invention relates to a manufacturing method and application of a nanoparticle biochip. The nanoparticle biochip is an immunoliposome compound nanoparticle biochip. The manufacturing method comprises the steps of: providing a substrate; letting the substrate surface and protein combine to form an active site by a surface treatment procedure; and carrying out reaction to make an antibody and theactive site to form a ligand receptor combination, thus obtaining the nanoparticle biochip.

Description

technical field [0001] The invention relates to a manufacturing method and application of a nanoparticle biochip, in particular to a manufacturing method and application of an immunoliposome complex nanoparticle biochip. Background technique [0002] Traditional biomolecular chip analysis techniques usually lack the characteristics of fast and simultaneous detection and analysis of various biological samples and molecules. For cell samples and extracellular vesicle samples, because the sample surface usually has a variety of membrane proteins or other receptors As a result, biochips need to perform complex sample pretreatment procedures before analysis, and then use biochips for analysis to achieve reliable analysis results. [0003] US Patent Publication No. US 20140094383 discloses a biochip. However, the biochip uses cationic nanomolecules to modify the surface of the biochip so that the biochip can have a better affinity with the negatively charged cell membrane due to e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/574G01N33/68
Inventor 李利郭广柱李沛恒
Owner SPOT BIOSYST LTD
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