A method for ultra-high pressure extraction of aescin from sage seeds based on freeze-drying

A kind of technology of aescin and aescin, which is applied in the direction of chemical instruments and methods, organic chemistry, steroids, etc., can solve the problems of increasing energy consumption and cost, difficulty in breaking cell walls, difficulty in penetrating solvents, etc., and achieve reduction in energy consumption and cost, reduce impurities, and reduce the effect of extraction cost

Active Publication Date: 2019-12-17
WUHAN AIMIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Sala seeds are hard, the cell wall is difficult to break, and the solvent is difficult to penetrate into the medicinal material tissue, resulting in the need for higher pressure (usually more than 500Mpa) and more times (usually more than 3 times of repeated extraction) during ultra-high pressure extraction, thereby increasing the Energy Consumption and Cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Crush the sara seeds into 15-mesh powder, add water to soak for 2 hours and then remove it, drain the surface water, and place it in a freezer at -25℃ to quickly freeze;

[0033] (2) Vacuum dry the frozen Salo seeds, control the vacuum degree of the drying process to be 10pa and the temperature to be 40°C;

[0034] (3) Add 12 times the weight of 20% ethanol to the dried salo seeds, put them in a pressure-resistant, flexible soft bag and seal them together, and then put them into the extraction container of the ultra-high pressure extraction device, and extract at room temperature. Proceed as follows:

[0035] 1) Pressure increasing stage: Raise the pressure in the extraction container to 200Mpa within 15 minutes;

[0036] 2) Pressure keeping stage: keep the pressure for 15 minutes;

[0037] 3) Pressure relief stage: quickly relieve the pressure to normal pressure within 10s;

[0038] 4) Repeat steps 1)-3) once;

[0039] (4) Take out the soft bag from the extraction container, ...

Embodiment 2

[0042] (1) Crush the sara seeds into powder of 30 mesh size, add water to soak for 3 hours and then remove it, drain the surface water, and place it in a freezer at -30℃ to quickly freeze;

[0043] (2) Vacuum dry the frozen Salo seeds, control the vacuum degree of the drying process to 20pa and the temperature to 50℃;

[0044] (3) Add 15 times the weight of water to the dried salo seeds, put them into a pressure-resistant, flexible soft bag and seal them together, and then put them into an ultra-high pressure extraction device, and extract at room temperature. The extraction steps are as follows:

[0045] 1) Pressure increasing stage: increase the pressure in the extraction container to 300Mpa within 10 minutes;

[0046] 2) Pressure holding stage: keep the pressure for 10 minutes;

[0047] 3) Pressure relief stage: quickly release the pressure to normal pressure within 15s;

[0048] 4) Repeat steps 1)-3) 2 times;

[0049] (4) Take the soft bag out of the ultra-high pressure extraction dev...

Embodiment 3

[0052] (1) Crush the sara seeds into powder with 10 mesh size, add water to soak for 1 hour and then remove it, drain the surface water, and place it in a freezer at -20℃ to quickly freeze;

[0053] (2) Vacuum dry the frozen Salo seeds, control the vacuum degree of the drying process to 15pa and the temperature to be 30°C;

[0054] (3) Add 10 times the weight of 30% ethanol to the dried salo seeds, put them in a pressure-resistant, flexible soft bag and seal them together, and then put them into the extraction container of the ultra-high pressure extraction device, and extract at room temperature. Proceed as follows:

[0055] 1) Pressure rising stage: Raise the pressure in the extraction container to 250Mpa within 15 minutes;

[0056] 2) Pressure holding stage: keep the pressure for 20 minutes;

[0057] 3) Pressure relief stage: quickly relieve the pressure to normal pressure within 5s;

[0058] 4) Repeat steps 1)-3) 3 times;

[0059] (4) Take the soft bag out of the ultra-high pressure ...

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Abstract

The invention discloses a method for ultra high-pressure extraction of escin from Chinese buckeye seeds based on freeze drying. The method includes: firstly crushing and soaking Chinese buckeye seed herbal material, then performing freeze drying, and then conducting ultra high-pressure extraction, wherein the extraction step includes a pressure boosting stage, a pressure maintaining stage, a pressure relief stage and the like. According to the invention, Chinese buckeye seeds are subjected to freeze drying treatment to change the internal structure of the herbal material texture, so that cellwalls can rupture more easily, and active ingredients can exude more easily, thereby reducing the pressure and number of times of ultra high-pressure extraction, and lowering energy consumption and cost.

Description

Technical field [0001] The invention belongs to the field of pharmacy, and relates to a method for extracting aescin, in particular to a method for extracting aescin from Salo seeds under ultra-high pressure based on freeze drying. Background technique [0002] Aescin, also known as aescinic acid, is the general term for total saponins, β-escin or isoscin, etc. extracted from the seeds of Aescinaceae, and belongs to the triterpene saponins. Aescin has many functions such as anti-inflammatory and anti-edema, and is mainly used for the treatment of cerebral edema, trauma and other diseases in clinic. At present, most of the extraction of aescin is the method of ethanol heat reflux or leakage. For example, CN 104402963 A, CN 102659897 A, CN 106589045 A, CN104804060 A and other patent documents all adopt this type of method. [0003] With the continuous advancement of traditional Chinese medicine extraction technology, more and more new methods are used in the extraction of the active...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/256C07J63/00C07H1/08
Inventor 石召华叶利春张晓存张群烁李群杜文杰张洋
Owner WUHAN AIMIN PHARMA
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