Liquid chromatographic detection method of polycyclic aromatic hydrocarbons in edible oil

Abstract

The invention discloses a liquid chromatographic detection method of polycyclic aromatic hydrocarbons in edible oil. The liquid chromatographic detection method comprises the following main steps: 1),extracting: weighing a proper amount of sample, putting into a clean centrifuge tube, dissolving the edible oil by using n-hexane saturated by acetonitrile, extracting the polycyclic aromatic hydrocarbons twice by using acetonitrile saturated by n-hexane, and transferring an acetonitrile layer to an eggplant-shaped bottle; 2), concentrating under reduced pressure: concentrating an extract under reduced pressure in a 40 DEG C water bath environment until the volume is about 2ml; 3), performing SPE purification: purifying an obtained concentrate by using an SPE column, and collecting an effluent in a graduated glass tube; 4), performing nitrogen blowing concentration: performing nitrogen blowing concentration on collected liquid at 40 DEG C until the volume is lower than 1ml; 5), fixing thevolume: fixing the volume by using acetonitrile until the volume is 1ml, filtering by using a syringe filter membrane with the thickness of 0.2 microns, and waiting for detection in a sample feedingbottle; 6), detecting and analyzing: analyzing and detecting an obtained solution to be detected by HPLC. By the liquid chromatographic detection method, the operation is simple and quick, the stability is good, the popularizing rate of detection equipment is high, and application is convenient.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a liquid chromatography detection method for polycyclic aromatic hydrocarbons in edible oil. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons PAHs) refer to compounds in which two or more benzene rings are connected in the form of fused rings, and are important environmental and food pollutants. Among the more than 200 kinds of polycyclic aromatic hydrocarbons that have been found so far, most of them are heavy polycyclic aromatic hydrocarbons with four to six rings. The International Cancer Research Center (IARC) (1976) lists 94 compounds that are carcinogenic to experimental animals. Among them, 15 kinds belong to polycyclic aromatic hydrocarbons. Since benzo[a]pyrene is the first environmental chemical carcinogen discovered, and its carcinogenicity is very strong, benzo[a]pyrene is often used as the rep...

AI Technical Summary

Problems solved by technology

The biggest disadvantage of this method is that the SPE column has poor retention of highly polar compounds such as naphthalene, acenaphthene, fluorene, and phenanthrene, and the recovery rate is very low or even no recovery.

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