A continuous/burst/phase-contrast three-mode ultrafast microscopic imaging method for real-time observation of micro-nano transient phenomena

Abstract

The invention discloses a continuous / burst / phase-contract three-mode ultrafast microscopic imaging method used for real-time observation of a micro-nano transient phenomenon, and specifically providesan optical path conversion module capable of changing the laser direction and enabling the femtosecond laser output by the same ultrafast pulse laser to pass through the optical path conversion module and enter the mode of continuous imaging or burst imaging for image processing. During observation of the same micro-nano transient phenomenon, the rapid dynamic characteristic in a micro-nano environment can be continuously captured, and the imaging that the time resolution capability of multiple frames achieves the femtosecond magnitude can be obtained at an important moment of an experiment.A phase detection module is additionally provided so that in the same transient phenomenon, the transparent portion of a sample can be observed, and the non-transparent portion of the sample can alsobe observed.

Description

technical field [0001] The invention relates to an ultrafast imaging technology, in particular to a continuous / burst / phase difference three-mode ultrafast microscopic imaging method for real-time observation of micro-nano transient phenomena. Background technique [0002] Traditional CCD and CMOS imaging are the most widely used imaging technologies. CCD and CMOS cannot clearly and sensitively capture the dynamic occurrence of ultrafast processes, especially for imaging objects at the micro-nano scale. Even if dynamic phenomena are slow, high temporal resolution is required at the micro- and nanoscale. [0003] A common problem with optical imaging is that the higher the frame rate, the fewer photons are collected per frame. In order to obtain a higher time resolution, the commonly used method is to reduce the spatial resolution and use a high-power light source, but this is not an ideal solution, not only will it generate optical noise, but also when performing micro-nano...

AI Technical Summary

Problems solved by technology

[0003] A common problem with optical imaging is that the higher the frame rate, the fewer photons are collected per frame

The human eye can only distinguish the wavelength and amplitude of visible light, but cannot distinguish the change of phase

Therefore, when the same observed object appears transparent and opaque at different time periods, only ultrafast imaging technology or phase contrast microscopy technology can no longer meet the observation requirements.

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