Primer and probe set and kit for detecting oncogene E6/E7 mRNA typing of high-risk human papilloma viruses

Abstract

The invention relates to a primer and probe set and a kit for detecting a virus, in particular to a primer and probe set and a kit for detecting oncogene E6 / E7 mRNA typing of high-risk human papillomaviruses, and belongs to the biochemical technology field. Specific upstream and downstream primers and corresponding specific molecular beacon probes are designed ingeniously for oncogenes E6 / E7 of thirteen high-risk HPVs (human papilloma viruses) and reference genes beta-actin, and specific amplification and high-specificity detection of the thirteen high-risk HPVs and the reference genes beta-actin are realized. The primer and probe set and the kit can be applied to real-time quantification and typing detection of oncogene E6 / E7 mRNA of the HPVs, and can be also applied to nature determination and typing detection of the thirteen HPVs at PCR (polymerase chain reaction) end points by the molecular beacon probes. The thirteen HPVs refer to HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59and 68. The oncogene E6 / E7 mRNA of the HPVs serves as a detection target, so that false positive results and false negative results both caused by HPV L1 DNA serving as a detection method in the prior art can be effectively avoided; the applied molecular beacon probes have the advantages of high detection sensitivity, high specificity, simplicity in operation and the like.

Description

technical field [0001] The invention relates to a primer, a probe and a kit for detecting a virus, in particular to a primer, a probe and a kit for detecting high-risk human papillomavirus E6 / E7 mRNA typing, and belongs to the technical field of biochemistry. Background technique [0002] Cervical cancer is one of the common malignant tumors in women. It is the only cancer whose cause has been found so far, which is caused by persistent infection of a single subtype of high-risk human papilloma virus (HPV). of. [0003] HPV belongs to the Papillomavacuovirus A genus of the Papovaviridae family. It is a spherical DNA virus that can cause squamous epithelial proliferation of human skin and mucous membranes. Studies have shown that more than 99% of cervical cancer is caused by HPV infection. HPV can be divided into epithelial type and mucosal type according to the infection site, among which the epithelial type includes types 1, 5, 8, 14, 20, 21, 25, 47, and the mucosal type ...

AI Technical Summary

Problems solved by technology

[0006] The object of the invention is to propose primers and probes and kits for detecting high-risk human papillomavirus E6 / E7 mRNA typing for the inaccurate detection results and low sensitivity of the prior art. The detection results have relatively high Good clinical reference value

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