Novel application of echinacoside

A technology of echinacoside and its application, applied in the new application field of echinacoside, can solve the problems of unable to prevent ESRD, backward action mechanism, etc.

Inactive Publication Date: 2018-02-06
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the treatment of diabetic nephropathy is mainly to control blood sugar, lower blood pressure, reduce urinary protein, control hyperlipidemia, low-protein diet, correct water and electrolyte metabolism disorders, etc. These treatments can only delay the progress of diabetic nephropathy, but cannot prevent the progression of ESRD at all. Traditional Chinese medicine has a long history in the treatment of diabetic nephropathy, but the research on its specific mechanism of action is relatively backward

Method used

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  • Novel application of echinacoside
  • Novel application of echinacoside
  • Novel application of echinacoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] [Implementation 1] Determination of mouse weight, blood sugar level and 24h urine collection and detection

[0039] (1) Fasting body weight of mice, blood collection from orbital sinus.

[0040] 1) Weight detection

[0041] ①Fasting: fast the experimental mice at 10:00 p.m.

[0042] ② Weighing: Weigh the mice at 8 weeks, 10 weeks, 12 weeks, 14 weeks, 16 weeks, and 18 weeks respectively, put an iron lunch box on the dynamic electronic balance, grab the mice, and put them into the weighing In the lunch box, weigh and record the data.

[0043] 2) The mice were anesthetized by intraperitoneal injection of pentobarbital, and blood was collected from the retroorbital vein, and the serum was separated.

[0044] ① Anesthesia: Prepare pentobarbital powder with normal saline to a concentration of 3%, intraperitoneally inject mice, and anesthetize mice at 0.1 mL / 10 g.

[0045] ② Routinely grab the mouse and put it on the blood collection platform. Grasp the skin between the tw...

Embodiment 2

[0063] [Implementation 2] Determination of BUN and Cr content in mouse serum

[0064] ① Take out the serum sample from the -80°C refrigerator, put it on ice quickly, and wait for the sample to melt at room temperature;

[0065] ②Centrifuge at 4000 rpm for 1 minute at room temperature to allow the serum on the wall of the EP tube to gather to the bottom of the tube.

[0066] ③ According to the operating procedure, turn on the automatic biochemical analyzer (Johnson&Johnson) and clean the injector.

[0067] ④ Put the EP tubes to be tested one by one according to the marking order of the sample trays of the automatic biochemical analyzer.

[0068] ⑤ Accurately install the reagent detection plate, and start using the automatic biochemical analyzer to detect the levels of BUN and Cr.

[0069] The evaluation indicators of the severity of diabetic nephropathy injury mainly include body weight, blood sugar, 24-hour urine protein content, blood BUN and blood Cr levels, and these indi...

Embodiment 3

[0071] [Example 3] Detection of kidney weight and kidney microstructure

[0072] 1. Terminal kidney tissue collection

[0073] When the mice were intervened to 10 weeks, weigh them, anesthetize the mice with 3% pentobarbital sodium, 0.1mL / 10g, fix them on the sampling board with a pillow, moisten the skin of the chest and abdomen of the mice with gauze, and cut open the chest cavity with ophthalmic scissors and the abdominal cavity to expose the heart and kidneys, cut the right atrial appendage, insert the needle of the syringe into the left ventricle, and slowly inject 10-15mL of PBS buffer with a 5050mL syringe. , keep only the kidney, peel off the capsule of both kidneys with tweezers, weigh them, put the right kidney into a 15mL centrifuge tube with 4% paraformaldehyde, put the left kidney into an EP tube, and store in a -80°C refrigerator save.

[0074] 2. Kidney tissue processing and pathological staining related experiments

[0075] 1) Kidney dehydrated, transparent,...

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Abstract

The invention provides application of echinacoside in the preparation of drugs for treating diabetic nephropathy. An experiment proves that ECH can significantly decrease body weight and blood glucoselevels, significantly reduce 24-hour urine protein content, significantly decrease kidney weight, BUN (blood urea nitrogen) and blood Cr levels, significantly reduce the content of renal interstitialfibrosis and the deposition of mesangial matrix, significantly decrease the expression level of alpha-SMA and increase the expression level of E-cadherin in the kidneys and significantly decrease theexpression levels of FN and Collagen IV in the kidneys.

Description

technical field [0001] The present invention relates to a new application of echinacoside. Background technique [0002] Diabetic nephropathy is one of the most common and serious complications of diabetes, which will eventually lead to terminal [0003] Early kidney disease (ESRD) is the most important cause of death in diabetic patients; in recent years, the prevalence of diabetes has been increasing year by year due to changes in people's lifestyles and eating habits; relevant epidemiological surveys show that adult diabetic patients will increase by 2010 285 million in 2030 will increase to 439 million in 2030, that is, by 2030, the number of diabetic patients in the world will more than double that of 2010. [0004] Diabetic nephropathy is clinically characterized by persistent albuminuria and / or progressive decline in glomerular filtration rate (GFR); its typical renal morphological changes include: glomerular basement membrane thickening, mesangial Matrix widening, ...

Claims

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Application Information

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IPC IPC(8): A61K31/7032A61P3/10A61P13/12
CPCA61K31/7032
Inventor 郝亚荣邱波唐凤娟张雪张祥蔡江
Owner WUHAN UNIV
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