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A kind of Tibetan medicine composition and its preparation method and application

A technology of composition and Tibetan medicine, applied in the directions of medicine combination, medicine formula, boron compound active ingredient, etc., can solve the problems of secondary pollution of grassland and water source, economic loss, high mortality, and achieve remarkable effect, easy operation, wide The effect of promoting application value

Active Publication Date: 2021-03-30
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tibet is one of the five major pastoral areas in the country. Its leading industry is yaks. Yaks are not only providers of production and living materials, but also direct labor in pastoral areas. Death, high mortality rate, not only brings huge economic losses, but also causes secondary pollution to pastures and water sources

Method used

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  • A kind of Tibetan medicine composition and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of preparation method of Tibetan medicine composition

[0017] 1 Materials and methods:

[0018] 1.1 Materials:

[0019] Herbs:

[0020] Scopolamine, Oxytropis verticillum, Acorus calamus, Borax, Gentiana quinquefolium, Pterostella, letter cone, turmeric, and melon seeds.

[0021] instrument:

[0022] ZDX-35BI seat type automatic electric heating pressure steam sterilizer, electronic balance (AL204, Mettler-Toledo Instruments Shanghai Co., Ltd.), digital constant temperature water bath (HH-2, Guohua Electric Co., Ltd.), induction cooker, GOODLOOK -1000 thin-layer chromatography imaging system (Shanghai Kezhe Biochemical Technology Co., Ltd.), electric heating constant temperature blast drying oven (DHG-9070, Shanghai Hongdu Electronic Technology Co., Ltd.), numerical control ultrasonic cleaner (KH7200DB, Kunshan Hechuang Ultrasonic Instrument Co., Ltd.), laboratory-specific ultrapure water machine, TDZ4-WS desktop low-speed centrifuge, Erlenmeyer flask, funne...

Embodiment 2

[0037] Antibacterial test of Tibetan medicine composition on in vitro

[0038] 1 Materials and methods

[0039] 1.1 Tibetan medicine composition and strains

[0040] The Tibetan medicinal composition consists of Scopolamine, Oxytropis verticillum, Calamus calamus, borax, Gentiana chinensis, Pterscapella, Xintongzi, turmeric, and melon seeds. The above medicinal materials were purchased from Tibet Yalang Tibetan Medicinal Materials Sales Co., Ltd.

[0041] Strains: Pasteurella multocida (No. CVCC 1659), purchased from China Veterinary Microbiological Culture Collection Management Center;

[0042] Medium: MH agar medium (Mueller-Hinton Agar, MHA), MH broth medium (Mueller-Hinton borth, MHB), both purchased from Beijing Aoboxing Biotechnology Co., Ltd.; LS4509 enrichment medium, batch number ZBAP -170716D, purchased from Guangzhou Dijing Microbial Technology Co., Ltd.

[0043] 1.2 Experimental animals

[0044] Kunming mice, half male and half female, weighing (20±2) g, were p...

Embodiment 3

[0068] In vivo antibacterial test of Tibetan medicine composition on Pasteurella:

[0069] 1 Materials and methods

[0070] 1.1 Tibetan medicine composition and strains

[0071] The Tibetan medicinal composition consists of Scopolamine, Oxytropis verticillum, Calamus calamus, borax, Gentiana chinensis, Pterscapella, Xintongzi, turmeric, and melon seeds. The above medicinal materials were purchased from Tibet Yalang Tibetan Medicinal Materials Sales Co., Ltd.

[0072] Strains: Pasteurella multocida (No. CVCC 1659), purchased from China Veterinary Microbiological Culture Collection Management Center;

[0073] Medium: MH agar medium (Mueller-Hinton Agar, MHA), MH broth medium (Mueller-Hinton borth, MHB), both purchased from Beijing Aoboxing Biotechnology Co., Ltd.;

[0074] 1.2 Experimental animals

[0075] Kunming mice, half male and half female, weighing (20±2) g, were purchased from the Experimental Animal Center of Lanzhou Veterinary Research Institute, Chinese Academy of ...

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Abstract

The invention discloses a Tibetan medicine composition, which is prepared from the following raw materials in parts by weight: 20-40 parts of root of hyoscyamus albus, 10-20 parts of herba spiranthislanceae, 10-20 parts of rhizoma calami, 1-2 parts of borax, 5-10 parts of radix gentianae marcrophyllae, 5-10 parts of herba pterocephali, 5-10 parts of radix embeliae, 5-10 parts of rhizoma curcumaelongae and 25-50 parts of melon seeds. The invention also provides a preparation method and application of the Tibetan medicine composition. The invention has the beneficial effects that the inventiondiscloses the Tibetan medicine composition, the preparation method of the Tibetan medicine composition, and use of the Tibetan medicine composition in prevention and treatment of hemorrhagic septicaemia caused by pasteurella multocida; Tibetan medicines are collocated and screened based on the Tibetan medicine theory and application rules as well as the traditional experience of handling on usingthe medicines and combining the technical methods such as modern immunopharmacology and clinical veterinary medicine; the Tibetan medicine composition is reasonable in formula, safe and effective, and easy in obtaining of the raw material; the preparation technology is simple and easy to operate; in vitro and in vivo efficacy test research proves that the Tibetan medicine composition is remarkable in effects and has wide clinical promotion and application value.

Description

technical field [0001] The invention relates to the technical field of Tibetan medicine compositions, in particular to a Tibetan medicine composition and its preparation method and application. Background technique [0002] Pasteurellosis multocida, also known as hemorrhagic septicemia, referred to as "cattle out beating", is an acute febrile septicemia caused by Pasteurella multocida and a variety of animals. Infectious diseases, in pastoral areas, are often sporadic or endemic. The pathogen is an opportunistic pathogen, which usually exists on the upper respiratory tract and digestive tract mucosa of healthy cattle. It does not cause disease under normal circumstances. When the body's resistance decreases Sometimes, under the action of adverse stress factors such as sudden changes in weather, long-distance transportation, and nutritional deficiencies, the germs can invade the body and multiply in large numbers. In addition to endogenous infection and disease, they can also...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9066A61P31/04A61P7/04A61K33/22
CPCA61K33/22A61K36/185A61K36/48A61K36/51A61K36/81A61K36/888A61K36/9066A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2300/00
Inventor 郝宝成高艳梁剑平拉巴次旦吴金措姆格桑卓嘎班旦四郎玉珍袁振杰
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS