A kind of preparation method of biogas fermentation promoting bacterial agent

A biogas fermentation and bacterial agent technology is applied in the field of biogas fermentation-promoting bacterial agent preparation, which can solve the problems of severe separation and cultivation, affecting popularization and application, and difficulty in obtaining bacterial strains, and achieves reduction of labor costs and time costs, low cost, and low cost. The effect of easy storage and transportation

Active Publication Date: 2020-11-03
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, the research in this field is not yet mature in terms of bacterial agent accelerators, and there is still a lot of room for improvement in the technical effects of gas production efficiency, gas production cycle and raw material adaptability
[0008] In addition, although some accelerators have good effects, the strains used are difficult to obtain or require rigorous isolation and cultivation, which affects their popularization and application.

Method used

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  • A kind of preparation method of biogas fermentation promoting bacterial agent
  • A kind of preparation method of biogas fermentation promoting bacterial agent
  • A kind of preparation method of biogas fermentation promoting bacterial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Fresh pig manure and rice straw powder with a weight ratio of 1:1 were used as fermentation substrates. The culture solution of each functional microorganism in the composite bacteria as inoculum is calculated by volume parts, respectively: the culture solution volume fraction of the rumen proteolytic bacteria is 8 parts, the culture fluid volume fraction of the syntrophomonas palmitic acid is 4 parts, the volume fraction of the culture solution of Thermobacterium faecalis is 8 parts, the volume fraction of the culture solution of Cellulophilus macroji is 10 parts, and the volume fraction of the culture solution of Proteobacterium glycolyticum is 10 parts, The volume fraction of the culture solution of Spirochete spirochete is 5 parts, the volume fraction of the culture fluid of Tunisia effluent toga is 5 parts, the volume fraction of the culture fluid of Enterobacter aerogenes is 5 parts, and the volume fraction of the culture fluid of Aminobacterium columbia is 5 part...

Embodiment 2

[0056] Fresh cow dung and rice straw powder with a mass ratio of 2:1 were used as fermentation substrates. The volume collocation ratios of each functional microorganism in the composite bacteria as the inoculum are respectively: the volume fraction of the culture solution of the rumen proteolytic bacteria is 5 parts, and the culture fluid volume fraction of the syntrophomonas palmitic acid is 5 parts. The volume fraction of the culture solution of Thermobacterium faecalis is 10 parts, the volume fraction of the culture solution of Cellulophilus gigantea is 12 parts, the volume fraction of the culture solution of Proteobacterium glycolyticum is 8 parts, and the cultivation of Spirochete spirochetes The volume fraction of the liquid is 5 parts, the volume fraction of the culture fluid of Tunisia effusions is 6 parts, the volume fraction of the culture fluid of Enterobacter aerogenes is 5 parts, and the volume fraction of the culture fluid of Aminobacterium Columbia is 5 parts, t...

Embodiment 3

[0060] Fresh pig manure and corn stalk powder with a mass ratio of 1:1 were used as fermentation substrates. The volume collocation ratio of each functional microorganism in the composite bacteria as inoculum is respectively: the volume fraction of the culture solution of the rumen proteolytic bacteria is 8 parts, and the culture fluid volume fraction of the syntrophomonas palmitic acid is 4 parts, and the The volume fraction of the culture solution of Thermobacterium faecalis is 7 parts, the volume fraction of the culture solution of Cellulophilus gigantea is 9 parts, the volume fraction of the culture solution of Proteobacter glycolyticum is 10 parts, and the cultivation of Spirochete spirochetes The volume fraction of the liquid is 5 parts, the volume fraction of the culture fluid of Tunisia effusions is 5 parts, the volume fraction of the culture fluid of Enterobacter aerogenes is 5 parts, and the volume fraction of the culture fluid of Aminobacterium Columbia is 4 parts, p...

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Abstract

The invention provides a preparation method of a microbial agent for promoting biogas fermentation. The preparation method includes: mixing an inoculum with a fermentation substrate, and performing anaerobic fermentation under 32-37 DEG C for 50 days to obtain the microbial agent, wherein the inoculum is prepared by mixing, based on the volumetric weight of microbial culture solutions, 15-20 partsof Firmicutes culture solution, 15-20 parts of bacteroid culture solution, 3-5 parts of spirochete culture solution, 4-6 parts of Thermotoga culture solution, 3-5 parts of mycetozoan culture solution, 3-5 parts of Synergistes culture solution, 3-5 parts of Fibrobacteres culture solution and 35-40 parts of Euryarchaeota culture solution; the fermentation substrate is agricultural organic waste. The preparation method has the advantages that the prepared microbial agent can satisfy the requirements of different types of raw materials, and the fermentation substrate of the microbial agent is lowin cost; the preparation method can greatly reduce manpower cost and time cost; the dry type anaerobic fermentation is used in the preparation process of the microbial agent, and the prepared microbial agent is low in water content and convenient to store and transport.

Description

technical field [0001] The invention relates to a preparation method of a biogas fermentation-promoting bacterial agent, which belongs to the technical field of organic waste utilization and renewable energy production. Background technique [0002] As a renewable resource of ground biomass, biogas has a long history of use. However, with the increasing demand for economical and scientific biogas production technology, many related technologies have been developed. Among them, the research on biogas accelerator is a hotspot. [0003] At present, the main accelerators can be simply divided into chemical substance accelerators and bacterial agent accelerators. [0004] The main research and development ideas of chemical substance accelerators are to promote the growth of methanogenic bacteria and improve their gas production efficiency, such as the Chinese patent with the authorization number CN 103642846 B and the Chinese patent with the publication number CN 102925493 A. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P5/02C02F11/04C12R1/01
CPCC02F11/04C12P5/023C12P39/00Y02E50/30
Inventor 李强尹小波马诗淳邓宇周正
Owner BIOGAS SCI RES INST MIN OF AGRI
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