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Method for rapid detection of Salmonella typhimurium based on photothermal effect of immunomagnetic nanomaterial

A Salmonella typhi and magnetic nanotechnology, applied in the field of immune detection, can solve the problems of many steps, the detection sensitivity needs to be improved, the time-consuming and the like, and achieve the effect of low cost and good practical application prospect.

Active Publication Date: 2018-02-09
山东师大瑞柏生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since most of these methods use visual or simple detection equipment, the sensitivity is still relatively low. Only by improving the sensitivity and adding pretreatment steps for enrichment and purification can it be suitable for the rapid screening of Salmonella in food samples with complex matrices.
[0004] From the above analysis, it can be seen that most of the current detection methods of pathogenic bacteria divide enrichment isolation-detection-test inactivation into three steps, which have many steps and take a long time, and most of the current reports on the analysis time only involve the detection of this method. However, the time required for the entire analysis process from sample processing to sample inactivation is not less than 2 hours, and if the enrichment process is required, it will not be less than 10 hours, and the detection sensitivity of existing pathogen detection methods still needs to be improved

Method used

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  • Method for rapid detection of Salmonella typhimurium based on photothermal effect of immunomagnetic nanomaterial
  • Method for rapid detection of Salmonella typhimurium based on photothermal effect of immunomagnetic nanomaterial
  • Method for rapid detection of Salmonella typhimurium based on photothermal effect of immunomagnetic nanomaterial

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Experimental program
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Embodiment

[0063] 1. Test materials and equipment

[0064] 1.1 Main materials and reagents

[0065] Table 1 Reagents and medicines

[0066]

[0067]

[0068]

[0069] 1.2 Main instruments and equipment

[0070] Table 3-2 Instruments and Equipment

[0071] Table 3-2Equipments and Instruments

[0072]

[0073]

[0074] 2 related solution preparation

[0075] Preparation of EDC / NHS solution: Weigh 9.6 mg of EDC and 9.6 mg of NHS and dissolve in 3 mL of secondary water.

[0076] 0.01mol·L -1 Phosphate buffer solution preparation: Weigh Na 2 HPO 4 12H 2 O 13.76g, NaH 2 PO 4 2H 2 O 1.79g, NaCl 9.00g, add deionized water to make up to 1000mL.

[0077] SYBR Green Ι standard solution preparation: Dilute the SYBR Green Ι stock solution 100 times with DMSO and store at -20°C.

[0078] Preparation of EDTA-2Na use solution: Weigh 18.61g of EDTA-2Na, add sterilized distilled water to make up to 100mL.

[0079] Preparation of LB liquid medium: Weigh 10 g of tryptone, 5 g of...

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Abstract

The invention discloses a method for rapid detection of Salmonella typhimurium based on the photothermal effect of an immunomagnetic nanomaterial. According to the method, a standard curve for the values of temperature rises and the numbers of Salmonella typhimurium is established by using the photothermal effect of an immunomagnetic nanomaterial, and the value of the temperature rise of a samplecan be substituted into the standard curve so as to obtain the number of Salmonella typhimurium in the sample; and the three links consisting of enrichment and separation, detection and sample inactivation are effectively integrated by using the magnetic and photothermal effect of the immunomagnetic nanomaterial. The method provided by the invention is sensitive, safe, rapid, portable and cheap; experimental results verify that the method has a minimal detection limit of 300 bacteria and the temperature rise value delta T is linearly related to the Salmonella typhimurium number in a range of 300-1000; therefore, the method provided by the invention has good practical application prospects and can be applied to actual rapid detection of Salmonella typhimurium.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a rapid detection method for Salmonella typhimurium based on the photothermal effect of immunomagnetic nanomaterials. Background technique [0002] The food safety problem caused by food-borne pathogens has always been a global problem that the World Health Organization and governments attach great importance to, and it is also one of the core and difficult points of food safety management and control. Salmonellosis is one of the important zoonotic diseases, and its pathogen Salmonella belongs to Enterobacteriaceae, which can cause food poisoning. [0003] The current detection methods for Salmonella generally require a multiplication step to increase the detectable rate of the pathogen, which is time-consuming and laborious. It takes 4 to 7 days to complete, and it is impossible to timely and quickly evaluate the content of Salmonella in food. At present, the immunolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/533
CPCG01N33/533G01N33/54326G01N33/54346G01N33/56916G01N2333/255G01N2446/90
Inventor 张鸿雁张震罗钰
Owner 山东师大瑞柏生物科技有限公司
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