Portable bacterial colony automatic counting device and method based on a smart phone

Abstract

The invention discloses a portable bacterial colony automatic counting device and method based on a smart phone. A lighting and control circuit board is arranged above a height adjusting plate. A light source diffusing plate is positioned above the lighting and control circuit board. A culture dish limiting plate is arranged above the light source diffusing plate. A bracket shell is arranged on the culture dish limiting plate. The culture dish limiting plate is provided with a through hole. A bacteriological culture dish is arranged in the through hole and on the light source diffusing plate.The bracket shell is provided with a smart phone placement position in which a smart phone is placed. The smart phone placement position is provided with a phone camera placement hole in which a camera is placed. The lighting and control circuit board provides backlight for the bacteriological culture dish to keep the bacteriological culture solution stable. The camera collects an image. The counting result is obtained by preprocessing the image through a specific algorithm. Bacterial colony counting operation commonly used in biochemical samples detection is realized. High accuracy and full automation are achieved at low cost. The device and the method can be conveniently used both in the laboratory and in the field.

Description

technical field [0001] The invention relates to the field of biochemical sample detection instruments, in particular to a portable automatic colony counting device and method based on a smart phone. Background technique [0002] Colony detection is one of the important methods for quality inspection in industries such as agriculture, food, medicine and hygiene, and quarantine inspection. Among them, the determination of the number of colonies is one of the basic daily tasks. At present, for the determination of the number of colonies, the national standard detection method, that is, the common nutrient agar pouring plate method to inoculate and count the colonies is still the so-called gold standard method with the highest reliability. After the colony culture is completed, the colonies on the Petri dish need to be counted. Traditional counting is done manually, which not only has high requirements for the experimenter himself, but even for experienced experimenters, it sti...

AI Technical Summary

Problems solved by technology

However, these commercial automatic colony counters are designed for laboratory applications, bulky and expensive, and are not suitable for use in the field and in resource-poor areas

[0004] In summary, the existing colony counting devices have a low degree of automation and high labor intensity. Although commercial products can achieve a high degree of automation, they are often expensive and bulky, making it difficult to popularize and apply them on a large scale. Suitable for field detection and use

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