High-efficient activated biological fermentation bait for aquatic product, and preparation method and application thereof

A bio-fermentation, high-efficiency technology, applied in biochemical equipment and methods, microorganism-based methods, applications, etc., to achieve the effects of simple preparation process, increased yield, and strong ability to withstand adverse environments

Inactive Publication Date: 2018-02-23
广东渔赞臣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the emergence of microecological preparations, enzyme preparations, Chinese herbal medicine preparations and alternatives to organic acid antibiotics have pla

Method used

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  • High-efficient activated biological fermentation bait for aquatic product, and preparation method and application thereof
  • High-efficient activated biological fermentation bait for aquatic product, and preparation method and application thereof
  • High-efficient activated biological fermentation bait for aquatic product, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0081] The embodiment of the present invention provides a method for preparing a high-efficiency active biological fermentation bait for aquatic products, comprising the following steps:

[0082] (1) The Bacillus subtilis is prepared by the following method:

[0083] Slant culture: slant medium prepared according to the following ratio: glucose 20g / L, peptone 15g / L, sodium chloride 1g / L, yeast extract 5g / L, agar 20g / L, pH 6.5; inoculate Bacillus subtilis Put it on the slant medium, and cultivate it for 30 hours at a temperature of 28°C to obtain the Bacillus subtilis species;

[0084] First-level seed culture: the first-level seed medium prepared in the following ratio: glucose 20g / L, peptone 15g / L, sodium chloride 1g / L, yeast extract 5g / L, pH 6.5; the resulting Bacillus subtilis species Inoculate into the primary seed culture medium, and cultivate for 30 hours at a temperature of 30°C and a rotational speed of 150 to 300 r / min to obtain a primary liquid seed culture solution...

Embodiment 2

[0102] Repeat step (1) in Example 1 of the present invention to obtain Bacillus subtilis; repeat step (2) in Example 1 of the present invention to obtain Bacillus coagulans; repeat step (3) in Example 1 to obtain saccharomyces .

[0103] The following raw materials are taken by weight percentage: imported fish meal 42%, fermented soybean meal 45%, small peptide 1%, Bacillus subtilis 2%, Bacillus coagulans 3%, yeast 3%, digestive enzyme 1%, organic acid 1.5% , growth-promoting factor 1.5% (wherein, described digestive enzyme is that cellulase, phytase, protease are mixed according to the weight ratio of 2:3:1; Described growth-promoting factor is vitamin E, copper sulfate, sulfurous acid Iron and manganese sulfate are mixed according to the weight ratio of 1:1:1:1); the Bacillus subtilis, Bacillus coagulans, and yeast are mixed into a composite bacterial agent, and then the obtained composite bacterial agent, imported fish meal, Fermented soybean meal, small peptides, digestiv...

Embodiment 3

[0105] Repeat step (1) in Example 1 of the present invention to obtain Bacillus subtilis; repeat step (2) in Example 1 of the present invention to obtain Bacillus coagulans; repeat step (3) in Example 1 to obtain saccharomyces .

[0106] The following raw materials are taken by weight percentage: imported fish meal 45%, fermented soybean meal 36%, small peptide 1.5%, Bacillus subtilis 3%, Bacillus coagulans 5%, yeast 5%, digestive enzyme 0.5%, organic acid 2% , growth-promoting factor 2% (wherein, described digestive enzyme is that cellulase, phytase, protease are mixed according to the weight ratio of 3:2:1; Described growth-promoting factor is vitamin E, copper sulfate, sulfurous acid Iron and manganese sulfate are mixed according to the weight ratio of 1:1:1:1); the Bacillus subtilis, Bacillus coagulans, and yeast are mixed into a composite bacterial agent, and then the obtained composite bacterial agent, imported fish meal, Fermented soybean meal, small peptides, digestiv...

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Abstract

The invention discloses a high-efficient activated biological fermentation bait for an aquatic product, and a preparation method and application thereof. The high-efficient activated biological fermentation bait for the aquatic product is prepared from the raw materials in percentage by weight: 30 to 45 percent of imported fish meal, 25 to 45 percent of fermented soybean meal, 0 to 30 percent of enzymolysis protein, 0.5 to 1.5 percent of small peptide, 1 to 5 percent of bacillus subtilis, 2 to 8 percent of bacillus coagulans, 0 to 5 percent of saccharomycetes, 0.1 to 1 percent of digestive enzyme, 0.5 to 2 percent of organic acid, and 0.5 to 2 percent of somatomedin. According to the high-efficient activated biological fermentation bait for the aquatic product, no any antibiotic is added,various cultures are organically combined, and flora advantages of the various cultures are fully played, so that not only can a digestive tract disease be prevented, but also fish and shrimp can be promoted to grow and gain weight, and the aim of complex synergism is achieved; in addition, the balance of the intestinal flora of the fish and shrimp can be improved and maintained, and the bait canbe directly applied to fish and shrimp cultivation.

Description

technical field [0001] The invention relates to the technical field of aquaculture feed, in particular to a high-efficiency active biological fermentation bait for aquaculture and its preparation method and application. Background technique [0002] At present, with the improvement of people's living standards, food quality and safety issues are increasingly valued by the country and the people, and pork and its organs are the first to bear the brunt of the Chinese people's traditional non-staple food. However, the residues of antibiotics in the organs of aquatic animals have also produced direct or indirect harm to human health. Therefore, reducing or stopping the use of antibiotics in feed has become an important task for researchers to solve the quality and safety of pork. In recent years, the emergence of microecological preparations, enzyme preparations, Chinese herbal medicine preparations and organic acid antibiotic substitutes have played a great role in solving thi...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/22A23K10/37A23K20/147A23K20/189A23K20/105A23K20/20A23K20/22A23K20/174A23K20/24A23K10/12C12N1/20C12N1/16C12R1/125C12R1/07
CPCA23K10/12A23K10/22A23K10/37A23K20/105A23K20/147A23K20/174A23K20/189A23K20/30A23K50/80C12N1/16C12N1/20Y02A40/818Y02P60/87
Inventor 王强张延涛王运功王臣臣
Owner 广东渔赞臣生物科技有限公司
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