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Immunizing peptide, method for producing immunizing peptide, pharmaceutical composition for immune disorders containing same, and method for treating immune disorders

一种医药组合物、免疫疾病的技术，应用在免疫疾病用医药组合物领域，能够解决难以获取抗体等问题

Inactive Publication Date: 2018-02-23
OSAKA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, from the problem of the huge number of combinations, it is difficult to obtain the antibody and use the antibody in therapy.

Method used

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  • Immunizing peptide, method for producing immunizing peptide, pharmaceutical composition for immune disorders containing same, and method for treating immune disorders
  • Immunizing peptide, method for producing immunizing peptide, pharmaceutical composition for immune disorders containing same, and method for treating immune disorders
  • Immunizing peptide, method for producing immunizing peptide, pharmaceutical composition for immune disorders containing same, and method for treating immune disorders

Examples

Experimental program
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Effect test

preparation example Construction

[0091] The method for preparing the peptide for immunization is not particularly limited, and examples thereof include chemical synthesis, decomposition of the target protein, and synthesis using recombinant DNA technology. In the case of the chemical synthesis method, the peptide for immunization can be synthesized by known organic synthesis using protecting groups such as benzyloxycarbonyl (Cbz), tert-butoxycarbonyl (Boc), fluorenylmethyloxycarbonyl (Fmoc), and the like. Method preparation. In the case of decomposition based on the target protein, the immunization peptide can be prepared, for example, by degrading the target protein using a known proteolytic enzyme such as protease or peptidase. The decomposition conditions of the target protein can be appropriately set according to, for example, the type of the target protein, the substrate specificity of the proteolytic enzyme, and the like. In the case of using the recombinant DNA technique, the immunization peptide, for...

Embodiment 1

[0131] It has been confirmed that the HEL protein is immunized and induces the complex of a peptide longer than the T cell epitope of the HEL protein and a living MHC class II molecule to generate antibodies, and the antibody inhibits the recognition of the T cell epitope / MHC class II molecule Binding of the TCR.

Embodiment 1A

[0133]It has been confirmed that immunization with HEL protein induces antibody production from a complex of a peptide longer than the T cell epitope of the HEL protein and a living MHC class II molecule.

[0134] (1) Preparation of HEL peptide prompt cells

[0135] From the mouse spleen cDNA will encode the following mouse MHC class Ⅱ molecules (I-A k ) polynucleotides of α-chain and β-chain were cloned on pME18S vector to prepare mouse MHC class Ⅱ molecules (I-A k ) α chain expression vector (I-A k α expression vector) and mouse MHC class Ⅱ molecules (I-A k ) β chain expression vector (I-A k β expression vector). In addition, pMX-GFP (acquired from the field of cell therapy, Center for Advanced Medical Research, Institute of Medical Science, The University of Tokyo) was used as the GFP expression vector.

[0136] Mouse MHC class II molecules (I-A k ) α chain (SEQ ID NO. 11)

[0137] ATGCCGCGCAGCAGAGCTCTGATTCTGGGGGTCCTCGCCCTGACCACCATGCTCAGCCTCTGCGGAGGTGAAGACGACATTGAGGC...

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Abstract

Provided is a novel immunizing peptide with which it is possible to induce antibody production to complexes that include the immunizing peptide and the MHC molecule of a living body. The immunizing peptide of the present invention is characterized in that: the immunizing peptide includes a T-cell epitope and antibody induction portion of a target protein; the antibody induction portion comprises one or more consecutive amino acids sequences upstream from the N-end amino acid of the T-cell epitope, and / or one or more consecutive amino acids sequences downstream from the C-end amino acid of theT-cell epitope, in the amino acid sequence of the target protein; and, when administered to a living body, the immunizing peptide induces antibody production to complexes that include the immunizing peptide and the MHC molecule of the living body.

Description

technical field [0001] The present invention relates to a peptide for immunization, a method for preparing the peptide for immunization, a pharmaceutical composition for immune diseases containing the same, and a method for treating immune diseases. Background technique [0002] It is well known that antibodies that recognize complexes of major histocompatibility complex (MHC) class II molecules and T cell epitopes presented by them inhibit T cell receptors specific for said complexes (hereinafter also referred to as "recognition T cell receptor of the complex", T cell receptor is also referred to as "TCR") recognizes the complex, and suppresses the onset of immune diseases and the like (Non-Patent Document 1). [0003] The antibody cannot be acquired by immunization of the T cell epitope, but requires a complex of the T cell epitope and the MHC class II molecule (hereinafter also referred to as "T cell epitope / MHC class II molecule") Immunization (non-patent literature 2-4...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00A61K38/00A61P37/04A61P37/06A61P37/08C07K14/47C07K14/74C12N15/09
CPCC07K14/47C07K14/465C07K14/7051C07K2319/00C07K2319/30A61P37/00A61K39/35A61K2039/55566A61K39/0008A61K2039/55588A61K2039/605C07K14/705A61K38/00C07K19/00C12N15/09G01N33/505G01N33/5052A61K39/39C07K16/2809
Inventor 荒濑尚岸田一辉
Owner OSAKA UNIV
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