Method for fast and quantificationally detecting plant salt resistant capability

A quantitative detection, plant technology, applied in the direction of testing plants/trees, measuring devices, material inspection products, etc., can solve the problems of long time, complex measurement process, no preventive effect, etc., to achieve the effect of less plant damage

Active Publication Date: 2018-03-02
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods are complicated to measure, have many indicators, take a long time

Method used

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  • Method for fast and quantificationally detecting plant salt resistant capability
  • Method for fast and quantificationally detecting plant salt resistant capability
  • Method for fast and quantificationally detecting plant salt resistant capability

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0030] Example 1: Take paper mulberry as an example. Healthy leaves with relatively consistent growth were picked on the campus of Jiangsu University for determination, and quickly and quantitatively tested the salt tolerance of Broussonetia papyrifera.

[0031] Step 1: Select fresh branches of the plant to be tested with leaves, and wrap the base of the branches with wet cotton to slow down the dispersion of water;

[0032] Step two, quickly return to the laboratory, and after cleaning the surface of the leaves, pick the leaves on the fresh branches with more consistent growth, and soak them in a basin filled with water for 1 hour;

[0033] Step 3: Prepare the reference salt solution (0.1Mol / L NaCl) and other test salt solutions (including 0.1Mol / L NaCl) 2 SO 4 And mixed salt solution); mix 0.1Mol / L NaCl, 0.1Mol / L Na 2 SO 4 Mixing is the mixed salt solution;

[0034] Step 4: After the leaves are soaked for 1 hour, take out the saturated leaves obtained after soaking, quickly and gent...

Example Embodiment

[0048] Example 2:

[0049] Take mulberry as an example to calculate its salt tolerance, and all steps are the same as in Example 1.

[0050] A capacitance sensor is used to measure the physiological capacitance C of each leaf of the mulberry tree at different times, and then the water potential W of the corresponding leaf tissue is measured with a water potential meter, as shown in Table 1. The leaf tension of the mulberry tree at different times after the saturated leaves are soaked in salt water is shown in the table 2; the relative salt resistance of mulberry tree to be tested salt solution is shown in Table 10.

[0051] Table 1 Physiological capacitance and tissue water potential of Broussonetia papyrifera and mulberry leaves at different times when saturated leaves are immersed in NaCl salt solution

[0052]

[0053] Table 2 Leaf tension of Broussonetia papyrifera and Mulberry at different times when saturated leaves are immersed in NaCl salt solution

[0054] time

[0055] Tab...

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Abstract

The invention discloses a method for fast and quantificationally detecting plant salt resistant capability, and belongs to the technical field of crop salt-resistant variety screening. The method hasthe advantages that a plant leaf stress degree and a plant leaf relative stress degree are both measured and calculated through a plant leaf water condition and physiological capacitance; then, the plant relative salt resistant capability is calculated through the leaf relative stress degree. The plant relative salt resistant capability is reflected through detecting plant electric physiological indexes; various defects of indexes in the prior art are overcome; in addition, high speed, simplicity and convenience are realized; the influence by natural environment is avoided; the salt resistancecapability of plants can be nondestructively and quantificationally detected.

Description

technical field [0001] The invention belongs to the technical fields of crop cultivation, salt-tolerant breeding, agricultural engineering and crop information detection, and in particular relates to a method for rapidly and quantitatively detecting the salt-resistant ability of plants. Background technique [0002] According to incomplete statistics, the global area of ​​saline-alkali land is about 1 billion hm 2 , accounting for about 7.6% of the world's land area. The total amount of saline-alkali soil resources in my country is about 99 million hm 2 , of which the modern saline-alkali soil area is 36 million hm 2 , the residual saline-alkali soil is about 45 million hm 2 , there are about 18 million hm 2 potential saline-alkali soil, and there is an increasing trend year by year. Therefore, how to utilize and develop salinized soil has become a very urgent demand in my country's agricultural production and improvement of the ecological environment. To develop and u...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 吴沿友黎明鸿于睿邢德科刘宇婧姚香平徐小健
Owner JIANGSU UNIV
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