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Method for fast and quantificationally detecting plant salt resistant capability

A quantitative detection, plant technology, applied in the direction of testing plants/trees, measuring devices, material inspection products, etc., can solve the problems of long time, complex measurement process, no preventive effect, etc., to achieve the effect of less plant damage

Active Publication Date: 2018-03-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are complicated to measure, have many indicators, take a long time, and are based on severe salt damage to crops, so they have no preventive effect

Method used

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  • Method for fast and quantificationally detecting plant salt resistant capability
  • Method for fast and quantificationally detecting plant salt resistant capability
  • Method for fast and quantificationally detecting plant salt resistant capability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Take building a tree as an example. The healthy leaves with relatively consistent growth were picked on the campus of Jiangsu University for determination, and the salt resistance ability of the mulberry tree was quickly and quantitatively detected.

[0031] Step 1, select the fresh branch of the plant to be tested with leaves, and wrap the base of the branch with wet cotton to slow down the water emission;

[0032] Step 2, quickly return to the laboratory, after cleaning the dust on the surface of the leaves, pick the leaves with relatively consistent growth on the fresh branches, put them in a basin filled with water and soak for 1 hour;

[0033] Step 3, prepare reference salt solution (the NaCl of 0.1Mol / L) and other salt solutions to be tested (comprising the NaCl of 0.1Mol / L) 2 SO 4 and mixed salt solution); the NaCl of 0.1Mol / L, the Na of 0.1Mol / L 2 SO 4 Mixing is a mixed salt solution;

[0034] Step 4: After the leaves are soaked for 1 hour, ta...

Embodiment 2

[0049] Taking mulberry as an example, calculate its salt tolerance, and all steps are the same as in Example 1.

[0050] The plant physiological capacitance C of each leaf of the mulberry tree was measured with a capacitance sensor at different times, and then the corresponding leaf tissue water potential W was measured with a water potential meter, as shown in Table 1; the leaf tension of the mulberry tree at different times after the saturated leaves were soaked in salt water 2; the relative salt tolerance of mulberry to be tested in salt solution is shown in table 10.

[0051] Table 1 Physiological capacitance and tissue water potential of mulberry leaves and mulberry leaves soaked in NaCl saline solution at different times

[0052]

[0053] Table 2 Leaf tension of mulberry and mulberry leaves soaked in NaCl salt solution at different times

[0054] time

mulberry tree

mulberry

0

46.483

43.817

2

178.905

71.648

4

290.64

...

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Abstract

The invention discloses a method for fast and quantificationally detecting plant salt resistant capability, and belongs to the technical field of crop salt-resistant variety screening. The method hasthe advantages that a plant leaf stress degree and a plant leaf relative stress degree are both measured and calculated through a plant leaf water condition and physiological capacitance; then, the plant relative salt resistant capability is calculated through the leaf relative stress degree. The plant relative salt resistant capability is reflected through detecting plant electric physiological indexes; various defects of indexes in the prior art are overcome; in addition, high speed, simplicity and convenience are realized; the influence by natural environment is avoided; the salt resistancecapability of plants can be nondestructively and quantificationally detected.

Description

technical field [0001] The invention belongs to the technical fields of crop cultivation, salt-tolerant breeding, agricultural engineering and crop information detection, and in particular relates to a method for rapidly and quantitatively detecting the salt-resistant ability of plants. Background technique [0002] According to incomplete statistics, the global area of ​​saline-alkali land is about 1 billion hm 2 , accounting for about 7.6% of the world's land area. The total amount of saline-alkali soil resources in my country is about 99 million hm 2 , of which the modern saline-alkali soil area is 36 million hm 2 , the residual saline-alkali soil is about 45 million hm 2 , there are about 18 million hm 2 potential saline-alkali soil, and there is an increasing trend year by year. Therefore, how to utilize and develop salinized soil has become a very urgent demand in my country's agricultural production and improvement of the ecological environment. To develop and u...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/0098
Inventor 吴沿友黎明鸿于睿邢德科刘宇婧姚香平徐小健
Owner JIANGSU UNIV
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