Method for detecting analgin, 4-(methylamino)antipyrine and caffeine in herbal tea
A technology of analgin and methylamino, which is applied in the field of detection of analgin, 4-antipyrine and caffeine in herbal teas, can solve problems such as difficulty in supervision and lack of fast and effective detection methods, and achieve good precision, The effect of fast detection speed and strong specificity
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[0024] 1) Preparation of sample solution: Ultrasonic extraction of solid herbal tea beverage with methanol solution, dilution of liquid herbal tea beverage with methanol solution, shake well, let stand, take supernatant and carry out microporous filtration to obtain sample solution;
[0025] 2) Preparation of the standard series of working solutions: first use methanol solution to prepare analgin standard stock solution, 4-(methylamino)antipyrine standard stock solution and caffeine standard stock solution with the same concentration, and then take an equal volume of three Mix the standard stock solutions and dilute them with methanol solution to prepare a mixed standard intermediate solution, then take several equal volumes of mixed standard intermediate solutions and dilute them to different multiples with methanol solution to obtain a standard series of working solutions;
[0026] 3) Preparation of the standard curve: inject the standard series of working solutions into the ...
Embodiment 1
[0037] A method for detecting Sulpyrine, 4-(methylamino)antipyrine and caffeine in herbal tea beverages, comprising the following steps:
[0038] 1) Preparation of sample solution: Measure 5mL of herbal tea beverage, add it to a 25mL volumetric flask, dilute to volume with methanol solution, mix evenly, let it stand still, take the supernatant and filter it with a microporous membrane with a pore size of 0.45 μm, and the filtrate is Sample solution; (concentration of methanol solution is 550g / L)
[0039] 2) Preparation of the standard series of working solutions: first use methanol solution to prepare Analgin standard stock solution, 4-(methylamino)antipyrine standard stock solution and caffeine standard stock solution with a concentration of 2000mg / L, and then take Three kinds of standard stock solutions of equal volume were mixed, diluted with methanol solution, and the mixed standard intermediate solution with a concentration (in terms of the concentration of Analgin) of 50...
Embodiment 2
[0052] A method for detecting Sulpyrine, 4-(methylamino)antipyrine and caffeine in herbal tea powder, comprising the following steps:
[0053] 1) Preparation of sample solution: Weigh 0.3g of herbal tea powder, put it into a 50mL volumetric flask, add 40mL of methanol solution, shake gently and mix well, then ultrasonically extract for 20min, after cooling to room temperature, use methanol solution to make up volume, mix well, and statically place, take the supernatant and filter it with a 0.45 μm microporous membrane, and the filtrate is the sample solution; (the concentration of the methanol solution is 550g / L)
[0054] 2) Preparation of the standard series of working solutions: first use methanol solution to prepare Analgin standard stock solution, 4-(methylamino)antipyrine standard stock solution and caffeine standard stock solution with a concentration of 2000mg / L, and then take Three kinds of standard stock solutions of equal volume were mixed, diluted with methanol solu...
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