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Body color character pure breed culture method of artificial induction gynogenesis golden fishes

A technology of gynogenesis and artificial induction, applied in fish farming, application, climate change adaptation, etc., can solve the problem of few stable genetic strains

Active Publication Date: 2018-03-13
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002]Goldfish (Carassius auratus) is currently the most common consumer species in the domestic ornamental fish market. Common body colors in the market include red, red and white, yellow, streaky, etc. But there are not many strains that can be stably inherited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Sperm inactivation treatment

[0027] The male fish were crucian carp with blue-gray body color, and the second injection method was used to induce production and ejaculation. The male broodstock was injected with a 2 μg luteinizing hormone releasing hormone analog (LHRH-A2) preparation needle for the first time; The weight of kg fish was injected with 5 mg compound ripening agent for the second injection. The crucian carp semen was collected routinely and diluted with 50 times Hank's solution to obtain the sperm dilution.

[0028] The formula of the compound ripening agent is that every 10 mg compound ripening agent contains 2 mg dioconone (DOM), 6 mg carp pituitary gland (PG), 800 international units IU human chorionic gonadotropin (HCG) .

[0029] Atmospheric room temperature plasma breeding instrument (ARTP) was used to repeatedly inactivate sperm dilutions in batches, and the total amount of inactivated sperm fluid from crucian carp was more than 300 μL; after...

Embodiment 2

[0044] 1. Sperm inactivation treatment

[0045] The male fish were crucian carp with blue-gray body color, and the second injection method was used to induce production and ejaculation. The male broodstock was injected with a 2 μg luteinizing hormone releasing hormone analog (LHRH-A2) preparation needle for the first time; The weight of kg fish was injected with 3 mg compound ripening agent for the second injection. Collect crucian carp semen according to routine, and use 40 times of Hank's solution to dilute to obtain sperm dilution.

[0046] The formula of the compound ripening agent is that every 10 mg compound ripening agent contains 2 mg dioconone (DOM), 6 mg carp pituitary gland (PG), 800 international units IU human chorionic gonadotropin (HCG) .

[0047] Use the Atmospheric Room Temperature Plasma Breeder (ARTP) to repeatedly inactivate sperm dilutions in batches. After treatment, add 5 μL 0.6M mannitol and 10 μL Hank’s solution to each 20 μL inactivated sperm fluid,...

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Abstract

The invention relates to a body color character pure breed culture method of artificial induction gynogenesis golden fishes. The method comprises the steps of adopting a secondary injection method toconduct parturition hastening and spermiation on caesious male carps; using a breeding apparatus of atmospheric and room temperature plasmas to conduct sperm inactivation treatment on a sperm diluentin batches; obtaining carp inactivation sperm fluid and completing fertilization with the carp inactivation sperm fluid and eggs generated by golden fishes of a target body color; adopting colchicine,combining a cold treatment mode, to treat fertilized ova of the golden fishes, and conducting gynogenesis diploidization and natural hatch; selecting F1-generation golden fishes with the target bodycolor character obtained through breeding; selecting F1-generation female golden fishes as parent fishes, selecting caesious carps as male fishes, and repeating the above steps to obtain F2-generationgolden fishes with the target body color character. According to the body color character pure breed culture method of the artificial induction gynogenesis golden fishes, F2-generation golden fish pure breed parents with the target body color character are used for breeding production, and a new fast path is established for targeted breeding of the target body color character of aquarium fishes.

Description

technical field [0001] The technology of the invention belongs to the technical field of goldfish artificial induction breeding, and in particular relates to a method for artificially inducing gynogenetic goldfish pure breed breeding method for body color traits. Background technique [0002] Goldfish (Carassius auratus) is currently the most common consumer species in the domestic ornamental fish market. Common body colors in the market include red, red and white, yellow, streaky, etc., but there are not many strains that can be stably inherited. Therefore, carrying out genetic research on the directional development of goldfish body color will not only greatly shorten the time for breeding high-quality color and improve breeding efficiency, but also create new economic value and have broad practical prospects. [0003] According to research data: foreign countries have made outstanding achievements in the research of body color selection and breeding of zebrafish, guppy, m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/10A01K2227/40Y02A40/81
Inventor 黄镇陈多岑万周翊涛黄祖新
Owner FUJIAN NORMAL UNIV
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