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Preparation method of feed capable of treating fish gill rot diseases

A technology for feed and gill rot disease, which is applied in the field of preparation of feed for fish gill rot disease, can solve the problems of drug resistance, short feed action time, and long-term protection, so as to promote growth, reduce stability, and resistance The effect of enhancing and restoring

Inactive Publication Date: 2018-03-27
李炫颖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: Aiming at the current problem of adding antibiotics to the feed for treating fish gill rot disease to prevent the disease, long-term use will produce drug resistance, and the feed for treating fish gill rot disease has a short action time and cannot provide long-term protection. A preparation method of feed for treating fish gill rot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] A preparation method for treating fish gill rot disease feed, comprising the steps of:

[0023] (1) Dry selenium-enriched oyster mushrooms, crush them, pass through an 80-mesh sieve, collect the sieved particles B, take the sieved particles A and sieved particles B, add distilled water and mix them at a mass ratio of 2:2:25, and adjust the pH to 5~ 5.5, add cellulase with 10~15% mass of sieved particle A, enzymolyze at 50~55℃ for 2~2.5h, inactivate the enzyme, take the inactivated enzyme solution and add distilled water at a mass ratio of 1:15, adjust the pH to 9.0~ 9.2, add alkaline protease with 20-30% mass of inactivating enzyme solution, extract at 50-55°C for 3-4 hours, centrifuge, take the supernatant, freeze-dry to obtain nutritional coarse powder, and set aside;

[0024] (2) Take fresh rhubarb and wash it, soak it in sterile water for 1-2 minutes, then soak it in 10% sodium hypochlorite solution for 10-15 minutes, take it out and rinse it with sterile water. Cu...

Embodiment 1

[0028]Pretreatment of saiteng fruit meal: take satoba fruit cake at 0.35MPa, steam explosion treatment for 120s, take the steam explosion product and add petroleum ether at a mass ratio of 1:6, reflux extraction at 70°C for 5h, filter, take the filtrate and rotary evaporate, Dry, crush and pass through a 100-mesh sieve, and collect the sieved particle A.

[0029] Rhubarb soaking juice: Weigh fresh rhubarb and add distilled water at a mass ratio of 1:10, keep at 95°C for 20 minutes, filter, and take the filtrate to obtain.

[0030] Fermentation medium: in parts by mass, take 150 parts of potato, 2 parts of glucose, 0.5 part of soybean oil, 10 parts of corn flour, 15 parts of rhubarb infusion, 1000 parts of water, and sterilize at 121°C for 20 minutes.

[0031] A preparation method for treating fish gill rot disease feed, comprising the steps of:

[0032] (1) Dry selenium-enriched Pleurotus ostreatus, crush them, pass through an 80-mesh sieve, collect the sieved particles B, ta...

Embodiment 2

[0037] Pretreatment of saiteng fruit meal: take satoba fruit cake at 0.35MPa, steam explosion treatment for 120s, take the steam explosion product and add petroleum ether at a mass ratio of 1:6, reflux extraction at 80°C for 6h, filter, take the filtrate and rotary evaporate, Dry, crush and pass through a 100-mesh sieve, and collect the sieved particle A.

[0038] Rhubarb soaking juice: Weigh fresh rhubarb and add distilled water at a mass ratio of 1:10, keep at 100°C for 25 minutes, filter, and take the filtrate to obtain.

[0039] Fermentation medium: in parts by mass, take 200 parts of potatoes, 5 parts of glucose, 0.8 parts of soybean oil, 13 parts of corn flour, 20 parts of rhubarb infusion, 1000 parts of water, and sterilize at 121°C for 20 minutes.

[0040] A preparation method for treating fish gill rot disease feed, comprising the steps of:

[0041] (1) Dry selenium-enriched Pleurotus ostreatus, crush them, pass through an 80-mesh sieve, collect the sieved particles ...

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Abstract

The invention discloses a preparation method of a feed capable of treating fish gill rot diseases, and belongs to the field of feeds. According to the preparation method disclosed by the invention, purified strains having restraining effects on aeromonas hydrophila and edwardsiella are extracted from fresh rheum officinale, and fermentation culture is performed on the purified strains, so that thefeed mainly restrains fish gill rot bacterium cell mitochondria from breathing and performing electron transfer, restrains the oxidization and dehydrogenation process of thalli sugar and sugar metabolism intermediate products, influences the supply of energy resources required for vital activity of tissue cells, and is combined with DNA to interfere template functions, restrain protein synthesisand nucleic acid synthesis, reduce biomembrane stability and interfere an enzymatic reaction of energy metabolism, the effects of restraining bacteria, sterilizing and diminishing inflammation can beachieved, and the feed can replace antibiotic type medicines for preventing diseases from being generated, and cannot cause the yield and the quality of fish to be reduced. Through the adoption of thepreparation method disclosed by the invention, the problems that antibiotics are added to a feed capable of treating fish gill rot diseases at present for preventing the fish gill rot diseases from being generated, after the feed is used for a long term, medicine resistance can be produced, the action time of the feed for treating the fish gill rot diseases is short, and the feed cannot protect the fish for a long term are solved.

Description

technical field [0001] The invention belongs to the field of feed, and in particular relates to a preparation method of feed for treating fish gill rot. Background technique [0002] my country is rich in freshwater fish resources, with more than 800 species. Among them, Cyprinidae has the most genera and species, accounting for about one-half of all freshwater fish; Siluridae and Loachidae account for about one-fourth of all freshwater fish; Account for a quarter of all freshwater fish. Among the freshwater fish in our country, grass carp, bream, silver carp, bighead carp, carp, crucian carp, etc. are widely distributed, and their delicious meat is deeply loved by the people. However, freshwater fish are extremely susceptible to gill rot during aquaculture. Gill rot is a relatively common fish disease with rapid infection and long course. The disease begins to occur and spread when the water temperature is above 15℃. It is the peak period of onset, mainly caused by Aero...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K20/195
CPCA23K20/195A23K50/80Y02A40/818
Inventor 李炫颖郑薇程依
Owner 李炫颖
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