A biostimulant formulation for improving plant growth and uses thereof
A technology for promoting plant growth and biostimulation, applied in the field of plant biology
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Embodiment 1
[0122] Isolation of Kappa heterocladum (sacol) seaweed into sap and pulp after extracting the sap
[0123] 1 kg of fresh Kappa heterocladum (sacol) seaweed was sourced from the Indonesian island of Makassar and processed through a laboratory centrifugal juicer. Centrifugal juicers have a perforated drum with tines. The whole seaweed comes into contact with the drum and immediately separates into juice and juice-extracted slurry without forming an intermediate slurry. After extracting the juice from seaweed, the slurry and seaweed juice are collected separately from two different outlets of the equipment in a continuous manner. The amount of juice extracted pulp collected was 350 g and the amount of juice collected was 650 g. The water content of the pulp after juice extraction was 80%, and the Brix of the juice was 5%.
Embodiment 2
[0125] Eucheuma seaweed isolated as juice and pulp after juice extraction
[0126] 1 kg of Eucheuma (Spinosum) was obtained from Zanzibar, Tanzania, packed in transparent plastic bags, and exposed to sunlight for 4-10 h. The juice leached from the biomass is collected in bags, leaving the whole algae intact and without forming any slurry. From 1 kg of fresh Spinosum, 800 g of juice-extracted pulp and 200 gm of juice were obtained.
Embodiment 3
[0128] Concentration of seaweed juice (SWJ)
[0129] 150ml of fresh Spinosum seaweed juice (about 4.5Bx) from Example 2 was concentrated to about 30ml using a rotary evaporator maintained under vacuum at 680mm Hg. The evaporating flask was kept at 65°C and the upright condenser was kept at 4°C by circulating water through the cooler. The Brix of the final concentrated juice is 22Bx. In another similar experiment, 1 kg of Kappa longcardia (cottonii) seaweed was separated into juice and slurry by the method in Example 1. 250ml of juice (about 5Bx) was concentrated to a final Bx of 25 in a rotary vacuum evaporator.
[0130] The concentrate juices of both Spinosum and cottonii were subjected to elemental analysis, which showed the following compositions (g / Kg) as shown in Table 1 below.
[0131] Table 1
[0132]
[0133]From the above analysis, it can be seen that regardless of the type of seaweed, potassium is the most abundant element present in the concentrated juice. I...
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