Mesenchymal stem cell-derived exosome

A stromal stem cell and stem cell technology, applied in the field of mesenchymal stem cell particles, can solve the problem of no report on the promoting effect of exosome corneal epithelial stem cell colony formation, and achieve the effect of increasing undifferentiated maintenance activity and promoting proliferation.

Active Publication Date: 2018-03-27
OSAKA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Furthermore, in recent years, focusing on the colony formation-promoting effect of corneal epithelial stem cells, studies on therapeutic agents for corneal epithelial stem cell deficiency, etc., have been conducted, but there is no report that exosomes have a colony-forming effect of corneal epithelial stem cells

Method used

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  • Mesenchymal stem cell-derived exosome
  • Mesenchymal stem cell-derived exosome
  • Mesenchymal stem cell-derived exosome

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Embodiment

[0123] Hereinafter, although an Example demonstrates this invention in more detail, this invention is not limited by these Examples at all.

[0124]

[0125] As adipose-derived mesenchymal stem cells (AD-MSCs), Poietics adipose-derived stem cells (PT-5006, Lonza) were used.

[0126]

[0127] Human corneal epithelial cells (HCEC) were prepared as follows. Human corneal epithelial cells were collected from the edge of human input cornea (Sight Life, WA), and used CnT-Prime, Epithelial Culture Medium (CnT-PR, CELLnTEC) in a 10 cm culture dish (353003, Falcon), at 37 ° C, 5 %CO 2 Cultivate in an incubator under a certain environment, and cultivate to a density of 70-90%. After washing once with 10 ml of PBS (14190-144, Gibco), use 2 ml of TrypLE Express (12605-010, Gibco) at 37°C, 5% CO 2 After carrying out the enzyme reaction in an incubator for 5 to 10 minutes, 8 ml of PBS was added, centrifuged at 1300 rpm for 5 minutes with a centrifuge (LC-230, TOMY), and the supernata...

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Abstract

The present invention relates to a mesenchymal stem cell-derived microparticle that has corneal epithelial stem cell and / or corneal epithelial cell growth-promoting activity, undifferentiated cornealepithelial stem cell maintenance activity, corneal epithelial stem cell colony formation-promoting activity, or a corneal epithelium protection effect.

Description

technical field [0001] The present invention relates to mesenchymal stem cell-derived microparticles having corneal epithelial stem cells and / or corneal epithelial cell proliferation-promoting activity, corneal epithelial stem cell undifferentiation-maintaining activity, or corneal epithelial stem cell colony-forming promoting activity, or corneal epithelial stem cells Epithelial protection. Background technique [0002] Corneal epithelial stem cells exist in the border between the cornea and conjunctiva, and play an important role in the cause and treatment of corneal epithelial stem cell deficiency, corneal epithelium disorder, and diabetic keratosis. Conventional treatments for these diseases include artificial tears, eye drops of antibiotics, and corneal transplantation, but there are cases where eye drops do not work, and allogeneic corneal transplantation has the risk of rejection and insufficient donors point. [0003] A method for inducing differentiation of iPS ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/28A61P27/02C12Q1/02
CPCC12Q1/02A61K35/12A61K35/28A61P27/02C12N5/0012C12N5/0663
Inventor 西田幸二林龙平本间阳一大久保彻柴田峻
Owner OSAKA UNIV
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