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Primers, kit and method to detect circular DNA circRNA_101835 and their application

A kit and circular technology, applied in the field of primers for detection of circular RNA circRNA_101835, can solve the problems of low sensitivity and specificity of serum gastric cancer markers

Inactive Publication Date: 2018-03-30
ZHENJIANG NO 1 PEOPLES HOSPITAL
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  • Abstract
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Problems solved by technology

However, the traditional serum gastric cancer markers (CEA, CA125, CA72-4, CA199) commonly used in clinic have low sensitivity and specificity
However, there is no report of circRNA in the peripheral blood of patients with gastric cancer

Method used

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  • Primers, kit and method to detect circular DNA circRNA_101835 and their application
  • Primers, kit and method to detect circular DNA circRNA_101835 and their application

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Embodiment Construction

[0042] The technical solutions of the present invention will be further described in detail below in conjunction with the drawings and embodiments of the specification.

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Abstract

The invention relates to primers, kit and method to detect circular DNA circRNA_101835 and their application. The primers to detect circular DNA circRNA_101835 are involved. The kit includes a blood sample RNA extracting reagent, the primers, a genome-removed DNA reaction system, a reverse transcription reaction system, and a qPCR (quantitative polymerase chain reaction) operation reaction system.The invention discloses expression of the circular DNA circRNA_101835 in people with gastric cancer and normal people and the use of fluorescence quantitative PCR to detect the circular DNA circRNA_101835. The circular DNA circRNA_101835 is suitable for assisted diagnosis of gastric cancer and evaluation on cancer treatment effect and prognosis condition.

Description

Technical field [0001] The invention uses a fluorescent quantitative PCR method to detect the abnormal expression and significance of circular RNA circRNA_101835 in the blood of gastric cancer patients, belongs to the technical field of molecular diagnosis, and relates to primers, kits, detection methods and applications for detecting circular RNA circRNA_101835. Background technique [0002] The incidence and mortality of gastric cancer in our country are both high. According to the China Cancer Registry Annual Report, the national incidence of gastric cancer is 36.21 per 100,000, ranking second in malignant tumors; its mortality rate is 25.88 per 100,000, ranking third in malignant tumors. The five-year survival rate of patients with advanced gastric cancer is less than 10% [1], and tumor recurrence and metastasis are the main causes of death. Therefore, the early diagnosis of gastric cancer and the control of metastasis are very important for the prognosis of patients. Howev...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 范钰郎亚昆柳迎昭陆上苏
Owner ZHENJIANG NO 1 PEOPLES HOSPITAL
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