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Preparation method of polylysine for bracket coating

A polylysine and coating technology, applied in coating, medical science, surgery, etc., can solve the problems of low drug release rate and short effective time, so as to improve release efficiency, prolong release time, and good biocompatibility The effect of sex and degradability

Inactive Publication Date: 2018-04-03
成都测迪森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing drug carriers generally include biodegradable and non-degradable polymer materials. In bioreduced polymer materials, the drug release rate is low and the effective time is short.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0020] Specific embodiment 1: a kind of polylysine for stent coating, preparation method is as follows:

[0021] Step 1. Insert the bacterial strain into the medium for cultivation, and cultivate it at 25-30° C. for 40 hours. The medium includes: 100 parts by weight of distilled water, 2 parts of glucose, 0.1 part of sodium chloride, 0.1 part of chlorine Calcium chloride: 0.1 part, ammonium chloride: 0.1 part, magnesium chloride: 0.1 part, and the pH value of the culture medium is 6.5.

[0022] Step 2. Inoculate the above-mentioned culture solution into the fermentation medium according to the inoculation amount of 5%, and cultivate it at 25-30° C. for 48 hours; the fermentation medium includes: 100 parts by weight of distilled water, 0.2 parts of peptone, yeast 0.2 parts of cream, 0.5 parts of glycerin, and 0.5 parts of glucose, and the pH value of the fermentation medium is 6.5.

[0023] Step 3, centrifuging the above fermented liquid for 20 minutes, collecting the cells, h...

specific Embodiment 2

[0025] Specific embodiment 2: a kind of polylysine for stent coating, preparation method is as follows:

[0026] Step 1. Insert the bacterial strain into the culture medium and culture it at 25°C for 40 hours. The culture medium includes: 100 parts by weight of distilled water, 2 parts of glucose, 0.1 part of sodium chloride, calcium chloride : 0.1 part, ammonium chloride: 0.1 part, magnesium chloride 0.1 part, the pH value of the medium is 6.5.

[0027] Step 2. Inoculate the above-mentioned culture solution into the fermentation medium according to the inoculum amount of 5%, and cultivate it at 25°C for 48 hours; the fermentation medium includes: 100 parts by weight of distilled water, 0.2 parts of peptone, and 0.2 parts of yeast extract part, 0.5 part of glycerol, 0.5 part of glucose, and the pH value of the fermentation medium is 6.5.

[0028] Step 3, centrifuging the above fermented liquid for 20 minutes, collecting the cells, heating in a water bath, taking the supernata...

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Abstract

The invention discloses a preparation method of polylysine for stent coating, which belongs to the medical field. The preparation method is as follows: step 1, inserting the bacterial strain into a medium for cultivation, and culturing at 25-30°C for 40 hours; Step 2. Inoculate the above-mentioned culture solution into the fermentation medium according to the inoculation amount of 5%, and cultivate it at 25-30°C for 48 hours; Step 3. Centrifuge the above-mentioned fermentation solution for 20 minutes, collect the cells, and heat them in a water bath. Take the supernatant, drain the water, and prepare polylysine; step 4: dissolve 2-amino-3-p-hydroxyphenylpropionic acid monomer and peroxide initiator in an organic solvent to prepare a solution; Secondly, it is mixed with the prepared polylysine powder, and the mixture is grafted and modified by melt grafting reaction equipment to obtain polylysine grafted 2-amino-3-p-hydroxyphenylpropionic acid biodegradable polymer.

Description

technical field [0001] The present invention relates to the field of stents. Background technique [0002] Cardiac stent (Stent), also known as coronary stent, is a medical device commonly used in cardiac interventional surgery and has the function of dredging arteries. The main materials are stainless steel, nickel-titanium alloy or cobalt-chromium alloy. [0003] Existing metal stents easily lead to restenosis of blood vessels. In a new type of stent, anti-thrombotic and anti-proliferative drugs are wrapped on the heart stent. After the stent is implanted, the drug is released slowly, which can increase the local drug concentration, reduce side effects, and reduce the rate of restenosis. [0004] Existing drug carriers generally include biodegradable and non-degradable polymer materials. In biodegradable polymer materials, the drug release rate is low and the effective time is short. Contents of the invention [0005] The object of the present invention is to: for abo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C08G69/48A61L31/10A61L31/14
CPCC12P13/02A61L31/10A61L31/14A61L31/148A61L2300/604A61L2300/606A61L2300/802C08G69/48C08L77/04
Inventor 郑浩
Owner 成都测迪森生物科技有限公司
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