Preparation method of polylysine for bracket coating
A polylysine and coating technology, applied in coating, medical science, surgery, etc., can solve the problems of low drug release rate and short effective time, so as to improve release efficiency, prolong release time, and good biocompatibility The effect of sex and degradability
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specific Embodiment 1
[0020] Specific embodiment 1: a kind of polylysine for stent coating, preparation method is as follows:
[0021] Step 1. Insert the bacterial strain into the medium for cultivation, and cultivate it at 25-30° C. for 40 hours. The medium includes: 100 parts by weight of distilled water, 2 parts of glucose, 0.1 part of sodium chloride, 0.1 part of chlorine Calcium chloride: 0.1 part, ammonium chloride: 0.1 part, magnesium chloride: 0.1 part, and the pH value of the culture medium is 6.5.
[0022] Step 2. Inoculate the above-mentioned culture solution into the fermentation medium according to the inoculation amount of 5%, and cultivate it at 25-30° C. for 48 hours; the fermentation medium includes: 100 parts by weight of distilled water, 0.2 parts of peptone, yeast 0.2 parts of cream, 0.5 parts of glycerin, and 0.5 parts of glucose, and the pH value of the fermentation medium is 6.5.
[0023] Step 3, centrifuging the above fermented liquid for 20 minutes, collecting the cells, h...
specific Embodiment 2
[0025] Specific embodiment 2: a kind of polylysine for stent coating, preparation method is as follows:
[0026] Step 1. Insert the bacterial strain into the culture medium and culture it at 25°C for 40 hours. The culture medium includes: 100 parts by weight of distilled water, 2 parts of glucose, 0.1 part of sodium chloride, calcium chloride : 0.1 part, ammonium chloride: 0.1 part, magnesium chloride 0.1 part, the pH value of the medium is 6.5.
[0027] Step 2. Inoculate the above-mentioned culture solution into the fermentation medium according to the inoculum amount of 5%, and cultivate it at 25°C for 48 hours; the fermentation medium includes: 100 parts by weight of distilled water, 0.2 parts of peptone, and 0.2 parts of yeast extract part, 0.5 part of glycerol, 0.5 part of glucose, and the pH value of the fermentation medium is 6.5.
[0028] Step 3, centrifuging the above fermented liquid for 20 minutes, collecting the cells, heating in a water bath, taking the supernata...
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