Selecting and breeding technology of stalk-wall-thickened lodging-resistant two-line male sterile line
A technique of sterile lines and stalks, which is applied in the field of breeding of two-line CMS lines with thickened stalk wall and lodging resistance, which can solve the problems of differences in lodging resistance, affecting rice quality, and yield reduction, etc.
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Embodiment 1
[0021] A kind of breeding technology of thickened stem wall and lodging resistance type two-line male sterile line, the steps of the breeding technology method are as follows:
[0022] (1) Selection of breeding materials. Rice-9311 was selected as the basic material for breeding, and then irradiated with cobalt-60r rays to obtain the mutant rice-9311 with thickened stalk wall obtained by radiation mutagenesis. The six-generation shuttle in the region is stable and named ZH001, using ZH001 as the raw material;
[0023] (2) Carry out breeding treatment to the mutant rice-931 after the mutagenesis in step 1, transplant the mutant rice-931 as a single plant, screen the variation single-plant listing marker without division, and pull out the sterile single-plant Heading out of the plant, select part of the fruiting mutant line rice for the next cultivation, so as to carry out the stability of the sterile mutant line for many years and the breeding of the sterile line, so as to obta...
Embodiment 2
[0033] A kind of breeding technology of thickened stem wall and lodging resistance type two-line male sterile line, the steps of the breeding technology method are as follows:
[0034] (1) Selection of breeding materials. Rice-9311 was selected as the basic material for breeding, and then irradiated with cobalt-60r rays to obtain the mutant rice-9311 with thickened stalk wall obtained by radiation mutagenesis. The six-generation shuttle in the region is stable and named ZH001, using ZH001 as the raw material;
[0035] (2) Carry out breeding treatment to the mutant rice-931 after the mutagenesis in step 1, transplant the mutant rice-931 as a single plant, screen the variation single-plant listing marker without division, and pull out the sterile single-plant Heading out of the plant, select part of the fruiting mutant line rice for the next cultivation, so as to carry out the stability of the sterile mutant line for many years and the breeding of the sterile line, so as to obta...
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