Activated sludge bacteria fixed cathode electrode of microbial fuel cell and preparation method thereof
A technology of activated sludge and anode electrodes, applied in biochemical fuel cells, battery electrodes, circuits, etc., can solve the problems of low MFC decontamination effect
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Embodiment 1
[0016] 1) Preparation of sludge bacteria
[0017] Take the anaerobic sludge from the sewage treatment plant, separate the colonies in the sludge by drawing a plate method, and culture them anaerobically at 30°C for 7 days; wash the colonies on the surface of the plate with the culture solution, and culture them anaerobically at 40°C for 3 days; Double the volume of culture solution, culture anaerobically at 38°C for 4 days, centrifuge to obtain viable sludge bacteria, add a small amount of culture solution, fully dissolve, store at 12°C for 10 hours, transfer to -2°C for 36 hours, and then vacuum freeze-dry. Instant sludge bacteria;
[0018] 2) Mixing
[0019] Mix 1 mg / ml of sludge bacteria, 5 mg / ml of quaternary ammonium salt, 3 mg / ml of conductive carbon black, 80 mg / ml of activated carbon and 10 parts of PVDF in 1) to obtain a mixed electrode material;
[0020] 3) Tablet
[0021] 2) The medium mixed electrode material is pressed into tablets by a tablet press with a pres...
Embodiment 2
[0023] 1) Preparation of sludge bacteria
[0024] Take the anaerobic sludge from the sewage treatment plant, separate the colonies in the sludge by drawing a plate method, and culture them anaerobically at 40°C for 3 days; wash the colonies on the surface of the plate with the culture solution, and culture them anaerobically at 38°C for 5 days; Double the volume of culture solution, culture anaerobically at 38°C for 5 days, centrifuge to obtain live sludge bacteria, add a small amount of culture solution, fully dissolve, store at 12°C for 12 hours, transfer to -2°C for 36 hours, and then vacuum freeze-dry. Instant sludge bacteria;
[0025] 2) Mixing
[0026] Mix 3 mg / ml of sludge bacteria, 15 mg / ml of quaternary ammonium salt, 5 mg / ml of conductive carbon black, 85 mg / ml of activated carbon and 10 parts of PVDF in 1) to obtain a mixed electrode material;
[0027] 3) Tablet
[0028] 2) The medium mixed electrode material was pressed into tablets with a tablet press, and the ...
Embodiment 3
[0030] 1) Preparation of sludge bacteria
[0031] Take the anaerobic sludge from the sewage treatment plant, separate the colonies in the sludge by drawing a plate method, and culture them anaerobically at 39°C for 6 days; wash the colonies on the surface of the plate with the culture solution, and culture them anaerobically at 40°C for 3 days; Double the volume of culture solution, culture anaerobically at 40°C for 3 days, centrifuge to obtain live sludge bacteria, add a small amount of culture solution, fully dissolve, store at 12°C for 10 hours, transfer to -2°C for 36 hours, and then vacuum freeze-dry. Instant sludge bacteria;
[0032] 2) Mixing
[0033] Mix 2 mg / ml of sludge bacteria, 10 mg / ml of quaternary ammonium salt, 4 mg / ml of conductive material, 80 mg / ml of activated carbon and 10 parts of PVDF in 1) to obtain a mixed electrode material;
[0034] 3) Tablet
[0035] 2) The medium mixed electrode material is pressed into tablets by a tablet press with a pressure ...
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