Compound type antibiotic-free feed additive

A technology of biological feed and compound microorganisms, which is applied in the field of animal feed, can solve problems such as the failure of bacteria to achieve good synergy, the inability to quickly form dominant bacteria, and the unreasonable combination of probiotics, so as to enhance animal immunity, The effect of promoting animal intestinal health and improving disease resistance

Inactive Publication Date: 2018-04-13
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are corresponding microecological preparation products at home and abroad, mainly some pure single or compound probiotic products, but these products have some shortcomings, which are mainly manifested in: 1. Poor temperature resistance, in transportation, storage, feed pelleting It is easy to be inactivated during the process, and the loss of live bacteria is large, which seriously affects the actual use effect of the product; 2. After use, the dominant flora cannot be formed quickly in the gastrointestinal tract of animals, and the actual effect is slow; 3. Probiotics The collocation is not reasonable, and good synergistic effects cannot be achieved between the strains; 4. When several types of products are used in combination, although the feeding effect is improved, the use cost per ton of full-price feed increases, which is much higher than that of adding antibiotics. Cost, economic benefits are very poorly competitive

Method used

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  • Compound type antibiotic-free feed additive
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  • Compound type antibiotic-free feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] In the present invention, the Oxford cup inhibition zone method is used to evaluate the inhibitory effect of different microorganisms on Escherichia coli.

[0032]1. Preparation of indicator bacteria suspension: inoculate Escherichia coli, Staphylococcus aureus, and Micrococcus luteus in the culture medium, culture in a shaking table at 37°C at 200r / min for 16-18 hours, and the bacterial concentration reaches 1.0-1.5× 10 10 CFU / mL.

[0033] 2. Preparation of lactic acid bacteria and bacillus suspensions: Inoculate pure strains in 100mL MRS liquid medium or nutrient broth medium under sterile conditions, and culture at 37°C for 24-36 hours, so that the bacterial concentration reaches 1.0-1.5 ×10 8 CFU / mL.

[0034] 3. Evaluation of antibacterial performance: Take the supernatant of the fermentation broth prepared in step 2, drop 200 μL into the Oxford cups on the plate, and incubate at 37°C for 18 hours, measure the diameter (or area) of each inhibition zone for evalua...

Embodiment 2

[0037] The preparation of embodiment 2 solid probiotics

[0038] Bacillus licheniformis was inoculated in liquid medium (containing mass meter, containing molasses 50g / L, bran 10g / L, corn steep liquor powder 5g / L, soybean meal powder 30g / L) and cultured at 36-37°C for 1-2 days, then transferred In the solid fermentation medium (molasses 3%, cornmeal: soybean meal: bran=2:3:1) by the transfer amount seed liquid of 10% (v / v), the water content of control solid fermentation medium is 50 %, cultured at 36-37°C for 2-3 days, the number of viable bacteria can reach 10 9 -10 10 CFU / g.

[0039] Pediococcus lactis was cultured at 36-37°C for 1-2 days with a liquid medium (6% molasses, 1% bran, 1% corn steep powder, and 1% soybean meal powder), and transferred 10% (v / v) seed liquid to In the solid fermentation medium (molasses 6%, corn flour: soybean meal: bran = 3:1:1), the water content of the solid fermentation medium is controlled to be 50%, cultivated at 36-37°C for 2-3 days, an...

Embodiment 3

[0042] The preparation of embodiment 3 ferment acidulant

[0043] Vegetables (cabbage, green vegetables, garlic, radish, etc.) stems and leaves are crushed and stirred, put into an enzyme bucket, add 20-30% sucrose accounting for vegetable mass, and inoculate commercially available fruit and vegetable enzyme powder at 10% (m / m) (contains yeast and lactic acid bacteria), ferment at room temperature for 30-40 days, and when the pH drops below 3, add secondary flour, corn flour, and wheat flour to the enzyme fermentation liquid to control the water content of the feed liquid at 40-50%, and continue After 10 days of fermentation, the fermented enzyme is dried at about 40° C. (35-45° C.) until the water content is below 10%, that is, a solid enzyme acidifier with pH ≤ 3 is obtained.

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Abstract

The invention discloses a compound type antibiotic-free feed additive and belongs to the field of animal feed. The feed additive disclosed by the invention is mainly prepared from a compound microorganism, a compound enzyme preparation, a mineral substance, oligosaccharide and echinacea polyphenol (2-6) and an acidifier. The antibiotic-free feed additive disclosed by the invention can be used forreplacing an antibiotic additive and the bacterium-inhibition effect is realized; the antibiotic-free feed additive can have the functions of promoting digestion and absorption of feed, inhibiting pathogenic bacteria, preventing diarrhea, promoting intestine health of animals, enhancing the immunity of the animals, preventing diseases and improving the disease-resisting capability. The compound type antibiotic-free feed additive is mainly added into the feed to carry out antibiotic-free feed breeding.

Description

technical field [0001] The invention relates to a composite antibiotic-free feed additive, which belongs to the field of animal feed. Background technique [0002] In the past two decades, my country's feed breeding industry has made great progress. Adding antibiotics to feed can inhibit pathogenic bacteria and prevent diseases; promote production: promote animal growth, increase daily weight gain, reduce feed-to-meat ratio, and improve animal feed utilization efficiency. The total output of antibiotics used in livestock and poultry farming has reached about 92,000 tons. The abuse of antibiotics or long-term use of antibiotics will cause great safety hazards to the entire ecological environment, making animal organisms dependent, limiting the function of immune cells in the body, and causing Animal immunity and disease resistance decline; antibiotics inhibit or kill harmful bacteria in the intestinal tract of animals, and at the same time inhibit or kill beneficial microorgani...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K10/12A23K10/30A23K20/189A23K20/163A23K20/28
CPCA23K10/12A23K10/18A23K10/30A23K20/163A23K20/189A23K20/28A23V2400/413
Inventor 余晓斌顾秋亚王丽杰方银兵叶盛群
Owner JIANGNAN UNIV
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