Glioma prognostic marker hsa_circ_0021827 and application

A diagnostic marker, glioma technology, applied in biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc., can solve the problem that the survival rate of glioma patients is not significantly improved, and the survival rate is not optimistic problems, to achieve the effect of improving clinical treatment effect, convenient and accurate diagnosis

Active Publication Date: 2018-04-20
XIANGYA HOSPITAL CENT SOUTH UNIV
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Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

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  • Glioma prognostic marker hsa_circ_0021827 and application
  • Glioma prognostic marker hsa_circ_0021827 and application
  • Glioma prognostic marker hsa_circ_0021827 and application

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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] The serum samples of 40 patients with glioma were provided by Xiangya Hospital, and the serum samples of 15 normal patients were healthy individuals undergoing community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0022] 2. Research Methods

[0023] 1. Extraction of RNA from Glioma / Normal Serum Exosomes

[0024] Take 200 μl of serum and centrifuge at 2000 g for 30 minutes at room temperature, use a micropipette to extract the supernatant into a new 600 μl centrifuge tube, add 40 μl of exosome extraction reagent (Total Exosome Isolation Reagent (from serum), product number 4478360, Invitrogen Company) gently Shake gently up and down, and incubate at 4°C for 45 minutes. After the...

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Abstract

The invention belongs to the biotechnology field, and discloses a glioma prognostic marker hsa_circ_0021827 and application. In the invention, it is firstly found that the expression level of hsa_circ_0021827 in the serum exosome of a glioma patient is obviously increased compared with a control group (p is equal to 0.025), and ROC analysis shows that the glioma prognostic marker hsa_circ_0021827has a relatively high diagnostic value for a glioma (AUC is equal to 0.823, P is equal to 0.003, the sensitivity is 70% and the specificity is 90.9%). Therefore, an early-stage rapid non-invasive diagnosis of the glioma patient can be made by detecting the expression level of hsa_circ_0021827 in the serum exosome of the glioma patient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker hsa_circ_0021827 for glioma diagnosis and the application of a reagent for detecting the marker to prepare glioma diagnostic preparations and a kit. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based on clinical, pathologica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 武明花周和诚李沛瑶冯剑波付海娟王维蒋健晖
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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