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Glioma diagnostic marker circ17:47618350|47619164 and its application

A technology of glioma and exosomes, which is applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems that the prognosis of glioma patients has not been significantly improved, and achieve improvement. Clinical treatment effect, convenient and accurate diagnosis

Active Publication Date: 2020-09-11
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although its treatment method has been developed from a single surgical treatment to today's comprehensive treatment such as surgery plus radiotherapy and chemotherapy, the prognosis of glioma patients has not been significantly improved in the past few decades.

Method used

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  • Glioma diagnostic marker circ17:47618350|47619164 and its application
  • Glioma diagnostic marker circ17:47618350|47619164 and its application
  • Glioma diagnostic marker circ17:47618350|47619164 and its application

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Embodiment Construction

[0019] The following embodiments are intended to further illustrate the present invention, rather than limit the present invention.

[0020] 1. Research object

[0021] The serum samples of 27 patients with glioma were provided by Xiangya Hospital, and the serum samples of 11 normal patients were healthy individuals undergoing community disease screening at the same time. The samples used for the research were collected at the same period, and the conditions of sampling, packaging and storage were the same.

[0022] 2. Research methods

[0023] 1. Extraction of RNA from Glioma / Normal Serum Exosomes

[0024] Take 200 μl of serum and centrifuge at 2000 g for 30 minutes at room temperature, use a micropipette to extract the supernatant into a new 600 μl centrifuge tube, add 40 μl of exosome extraction reagent (Total Exosome Isolation Reagent (from serum), product number 4478360, Invitrogen Company) gently Shake gently up and down, and incubate at 4°C for 45 minutes. After the...

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Abstract

The invention belongs to the biotechnology field, and discloses a glioma prognostic marker circ17:47618350|47619164 and application. In the invention, it is firstly found that the expression level ofcirc17:47618350|47619164 in the serum exosome of a glioma patient is obviously increased compared with a control group (p is equal to 0.0218), and ROC analysis shows that the glioma prognostic markercirc17:47618350|47619164 has a relatively high diagnostic value for a glioma (AUC is equal to 0.882, P is less than 0.005, the sensitivity is 79.2% and the specificity is 100%). Therefore, an early-stage rapid non-invasive diagnosis of the glioma patient can be made by detecting the expression level of circ17:47618350|47619164 in the serum exosome of the glioma patient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker circ17:47618350|47619164 for glioma diagnosis, and the application of a reagent for detecting the marker to prepare a glioma diagnostic preparation, and a kit. Background technique [0002] Gliomas originate from glial cells in the brain and are common intracranial tumors, accounting for about 40% to 50% of central nervous system tumors, of which malignant gliomas (World Health Organization classification III, IV) account for about 77.5% of all gliomas. Glioma has the characteristics of "three highs and one low", that is, high incidence, high postoperative recurrence, high mortality and low cure rate. resection. Although the treatment method has been developed from a single surgical treatment to today's comprehensive treatment such as surgery plus radiotherapy and chemotherapy, the prognosis of glioma patients has not been significantly improved in the past few...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 刘洋武明花李沛瑶刘涛付海娟冯剑波张严
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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