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ELISA method of transcriptomics and proteomics in biological process of liver cancer

A proteomics, biological technology, applied in the field of gene transcriptomics and proteomics

Inactive Publication Date: 2018-04-20
南宁科城汇信息科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0009] The ELISA of transcriptomics and proteomics in the biological process of liver cancer is an indispensable part of this research. The technical scheme of the ELISA method of transcriptomics and proteomics in the biological process of liver cancer of the present invention has been searched domestically. The same industry has not seen the same

Method used

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Embodiment

[0025] This ELISA method for transcriptomics and proteomics in liver cancer biology,

[0026] Consists of the following steps:

[0027] ① Dilute the antibody with coating buffer to a protein content of 1-10 μg / ml;

[0028] ②Add 0.1ml to the reaction well of each polystyrene plate, overnight at 4°C;

[0029] ③The next day, discard the solution in the well, and wash 3 times with washing buffer, 3 minutes each time;

[0030] ④ Add 0.1ml of a certain dilution of the sample to be tested to the above-mentioned coated reaction wells, incubate at 37°C for 1 hour and then wash (note that blank wells, negative control wells and positive control wells are reserved);

[0031] ⑤ Add 0.1ml of freshly diluted enzyme-labeled antibody (diluted after titration) to each reaction well;

[0032] ⑥Incubate at 37°C for 0.5-1 hour, then wash;

[0033] ⑦Add 0.1ml of temporarily prepared TMB substrate solution to each reaction well, and keep at 37°C for 10-30 minutes;

[0034] ⑧Add 0.05ml of stop ...

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Abstract

The invention discloses an ELISA method of transcriptomics and proteomics in a biological process of liver cancer. The method comprises the steps: (1) diluting an antibody with a buffer solution; (2)allowing the diluted antibody to stay overnight in each reaction hole of a polystyrene board; (3) next day, discarding the solution in the holes, and washing and buffering; (4) adding a certain diluted to-be-tested sample into the reaction holes, and then washing; (5) adding a fresh diluted enzyme labeled antibody in each reaction hole; (6) incubating and washing; (7) adding temporarily prepared TMB into each reaction hole; (8) adding a stop solution in each reaction hole; and (9) judging a result. A clinical sample is adopted for positioning quantitative expression verification, an evidence of the clinical sample with clinical relevance is searched, clinical value is evaluated, and a new clue is provided for research of liver cancer pathogenesis and a liver cancer mechanism. Screened keymolecules of liver cancer can lay the research basis for exploration of early detection, classification, evaluation of prognosis relevant liver cancer markers, and choice of more effective and accurate liver cancer treatment target sites.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to an ELISA method for transcriptomics and proteomics in the biological process of liver cancer. Background technique [0002] One of the most important fields in biological and medical research is the observation of the structure, function and regulation of biological systems and life processes. However, in the past few hundred years, biologists have been focusing on the expression changes and functions of individual genes or proteins in biological systems, but cannot study the changes in living systems from a global and holistic perspective. With the advancement of medicine, people have found that many diseases, especially cancer, are often caused by the synergistic effects of multiple factors, multiple genes, and multiple pathways. This requires a technology and means that can comprehensively, dynamically and systematically study the life system, so the concep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57438
Inventor 于思创王海云
Owner 南宁科城汇信息科技有限公司
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