Method for separating benzoic acid, sorbic acid, salicylic acid and phenoxyethanol and analyzing contents of benzoic acid, sorbic acid, salicylic acid and phenoxyethanol in toothpaste

Abstract

The invention relates to detection and analysis of a toothpaste preservative, and concretely relates to a method for separating benzoic acid, sorbic acid, salicylic acid and phenoxyethanol and analyzing contents of the benzoic acid, the sorbic acid, the salicylic acid and the phenoxyethanol in a toothpaste. The method comprises the following steps of (1) utilizing a high performance liquid chromatography for drawing a standard work curve of the benzoic acid, the sorbic acid, the salicylic acid and the phenoxyethanol; (2) weighing a toothpaste sample, using a methanol aqueous solution for dissolving, stirring and ultrasonically dissolving, and then sizing to obtain a sample solution; (3) after centrifuging the sample solution in the step (2), filtering a liquid supernatant through a microfiltration membrane to obtain a liquid to be tested; (4) using the high performance liquid chromatography for testing the liquid to be tested in the step (3), obtaining peak areas of the benzoic acid, the sorbic acid, the salicylic acid and the phenoxyethanol in the toothpaste sample, and obtaining the contents of the benzoic acid, the sorbic acid, the salicylic acid and the phenoxyethanol in the toothpaste sample through the standard work curve in the step (1). The method provided by the invention can be used for completely separating the benzoic acid, the sorbic acid, the salicylic acid and the phenoxyethanol, and has the characteristics of high column efficiency, good selectivity, good repeatability, wide linearity range, high detection limit, simplicity in operation, and the like.

Description

technical field [0001] The invention relates to the detection and analysis of toothpaste preservatives, in particular to a method for the separation and content analysis of benzoic acid, sorbic acid, salicylic acid and phenoxyethanol in toothpaste. Background technique [0002] In recent years, food safety issues have emerged in an endless stream, which has aroused widespread concern in the society. Subsequently, some scholars and public opinion turned their perspective to oral care products, and toothpaste, as a representative of oral care products, is related to personal safety issues. Therefore, the content and safety supervision of various additives in toothpaste are particularly important. Benzoic acid, sorbic acid, salicylic acid, and phenoxyethanol are used as preservatives and added to toothpaste to delay the growth of microorganisms or the corruption caused by chemical changes. [0003] Benzoic acid is a scaly or needle-like crystal with the smell of benzene or fo...

AI Technical Summary

Problems solved by technology

[0005] Chinese patent (Application No. 201110168425.X) uses methanol and ammonium acetate as the mobile phase, and adopts three-wavelength channel isocratic elution to separate preservatives benzoic acid, sorbic acid, and salicylic acid. Although the analysis time of this patent is relatively short, it uses three Channel for analysis and detection has high requirements for analytical instruments and is not suitable for high-throughput analysis of multiple indicators in samples; Chinese patent (application number 201510484674.8) uses methanol, acetonitrile, water, and sodium dihydrogen phosphate as four solvents to form the mobile phase. Gradient elution at room temperature for 40 minutes to separate phenoxyethanol and benzoic acid in chemicals, although this method has a good detection limit, but this method uses four-phase mobile phase mixing for analysis, which interferes with the analysis of actual samples larger

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