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Preparation method and application of Pseudomonas genus microorganisms and biomass carbon-based composite materials

A technology of Pseudomonas genus and composite materials, applied in the field of water treatment

Active Publication Date: 2021-02-26
HUNAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problem of efficient removal of heavy metal pollutants in sewage, the present invention provides a preparation method and application of Pseudomonas genus microorganisms and biomass carbon-based composite materials for adsorption and removal of heavy metals

Method used

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  • Preparation method and application of Pseudomonas genus microorganisms and biomass carbon-based composite materials
  • Preparation method and application of Pseudomonas genus microorganisms and biomass carbon-based composite materials
  • Preparation method and application of Pseudomonas genus microorganisms and biomass carbon-based composite materials

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preparation example Construction

[0027] A preparation method of the above-mentioned biomass carbon-based composite material, comprising the following steps:

[0028] Pseudocamarosporium sp. ZNT-10 (Pseudocamarosporium sp. ZNT-10) was placed at 25°C in the presence of Fe 3+ or Mn 2+ Cultured in PDA medium for 3 to 5 days.

[0029] Collected and adsorbed Fe 3+ or Mn 2+ The Pseudocamarosporium sp. ZNT-10 (Pseudocamarosporium sp. ZNT-10) was fired at 200°C-450°C for 0.5h-1.5h under protective gas to obtain a biomass carbon-based composite material.

[0030] If the culture temperature of Pseudocamarosporium sp. ZNT-10 is too high or too low, it will grow slowly, which is not conducive to producing a large number of microorganisms. Pseudomonas shield ZNT-10 (Pseudocamarosporium sp. ZNT-10) culture time is less than 3 days, Fe 3+ and / or Mn 2+ Insufficient adsorption, Fe adsorbed on the surface of Pseudocamarosporium sp. ZNT-10 for more than 5 days 3+ and / or Mn 2+ Desorption occurs.

[0031] Collected and ad...

Embodiment 1

[0041] (1) Pseudocamarosporium sp. ZNT-10 (Pseudocamarosporium sp. ZNT-10) was amplified and cultured in PDA medium for 3 days at 25°C;

[0042] (2) Pseudocamarosporium sp.ZNT-10 (Pseudocamarosporium sp.ZNT-10) at 25°C, in the presence of 150mg / L FeCl 3 Cultivated in the PDA medium for 4 days;

[0043] (3) Collect and adsorb Fe 3+ Pseudocamarosporium sp. ZNT-10 of Pseudocamarosporium sp. ZNT-10;

[0044] (4)N 2 Under protective conditions, burn at 450°C for 1.5h to prepare biomass carbon-based composite materials;

[0045] (5) Put 1g of biomass carbon-based composite material into 1L of Cd-containing 2+ 25mg / L of sewage, after 3h, filter the biomass carbon-based composite material, take 1mL of the supernatant, dilute 25 times, and use the atomic spectrophotometer method to detect the Cd in the supernatant 2+ Content, Cd 2+ The content is 0mg / L, after conversion, the removal rate reaches 100%.

Embodiment 2

[0047] (1) Pseudocamarosporium sp. ZNT-10 (Pseudocamarosporium sp. ZNT-10) was amplified and cultured in PDA medium for 5 days at 20°C;

[0048] (2) Pseudocamarosporium sp.ZNT-10 (Pseudocamarosporium sp.ZNT-10) at 20°C, in the presence of 300mg / L FeCl 3 Cultured in PDA medium for 3 days;

[0049] (3) Collect and adsorb Fe 3+ Pseudocamarosporium sp. ZNT-10 of Pseudocamarosporium sp. ZNT-10;

[0050] (4)N 2 Under protective conditions, burn at 400°C for 1 hour to prepare biomass carbon-based composite materials;

[0051] (5) Put 1g of biomass carbon-based composite material into 0.5L containing Cd 2+ 50mg / L of sewage, after 2h, filter the biomass carbon-based composite material, take 1mL of the supernatant, dilute it 25 times, and use the atomic spectrophotometer method to detect the Cd in the supernatant 2+ Content, Cd 2+ The content is 0.034mg / L, after conversion, the removal rate reaches 98.28%.

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Abstract

A preparation method and application of a Pseudocamarosporium microorganism and a biomass carbon-based composite material. The Pseudocamarosporium microorganism is Pseudocamarosporium sp. ZNT‑10 (Pseudocamarosporium sp. Collection Center, the deposit number is CCTCC M 2017468. The above-mentioned Pseudocamarosporium sp. ZNT‑10 (Pseudocamarosporium sp. ZNT‑10) has simple culture conditions, fast growth, low cost, large biomass, no environmental ecotoxicity, and high Mn and high Cd resistance. It and Fe 3+ and / or Mn 2+ Combined preparation of biochar-based composites can effectively remove Cd in water 2+ .

Description

technical field [0001] The invention relates to the field of water treatment, in particular to the preparation method and application of Pseudomonas genus microorganisms and biomass carbon-based composite materials Background technique [0002] With economic development, population growth, industrial and mining development, and the acceleration of urbanization, the demand for production and domestic water is increasing day by day, sewage discharge is increasing day by day, and the contradiction between supply and demand of water resources is becoming increasingly prominent. Sewage treatment is of great significance to effectively alleviate the shortage of water resources in my country, alleviate the shortage of industrial, mining and domestic water, improve the efficiency of water resource utilization, and ensure the safety of water supply. Affected by factors such as the production cost of sewage treatment and the technical level of sewage treatment plants, various toxic an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F3/34C01B32/05C01G45/02C01G49/06C12R1/645
CPCC01G45/02C01G49/06C01P2004/80C02F3/34C02F2101/20C12N1/145C12R2001/645
Inventor 陈章冯涛郑治建王慧敏刘涛邵琪陈国梁李志贤陈远其
Owner HUNAN UNIV OF SCI & TECH