SEC61A1 gene-containing detection kit for improving Altay sheep meat production performance

A technology of SEC61A1 and kit, which is applied in the identification field of animal genetic selection and breeding, and can solve the problems of affecting signal peptide orientation, no Altay mutton quality, unstable pore structure permeability, etc.

Inactive Publication Date: 2018-05-04
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mutation of some single nucleotides in the SEC61A1 gene can lead to an increase in the flexibility of some amino acid residues, thereby destabilizing the pore structure of the channel and changing the permeability, affecting translocation or inducing abnormal signal peptide orientation
At present, the research on this gene is mainly focused on human diabetes and tubulointerstitial nephritis and other diseases, and there is no report related to the quality of Altay mutton

Method used

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  • SEC61A1 gene-containing detection kit for improving Altay sheep meat production performance
  • SEC61A1 gene-containing detection kit for improving Altay sheep meat production performance
  • SEC61A1 gene-containing detection kit for improving Altay sheep meat production performance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kit containing the SEC61A1 gene for improving the meat production performance of Altay sheep. The kit was obtained through the establishment of the following co-construction system:

[0031] (1) Design primer pair: using the mRNA sequence of sheep SEC61A1 gene (GenBank accession number: SEC61A1:XM_015102503.1), artificially design specific primers, the primers are as follows:

[0032] SEC61A1 primer:

[0033] Upstream primer F: 5'-TATGTGATGACGGGAATGT-3';

[0034] Downstream primer R: 5'-GGTCTCGCAGATGTTAGTG-3'.

[0035] (2) PCR reagents: Mix, upstream and downstream primers, cDNA template and RNase-free water.

Embodiment 2

[0037] The application of the kit containing the SEC61A1 gene for improving the meat production performance of Altay sheep comprises the following steps:

[0038] (1) Genomic RNA extraction:

[0039] Collect 30-50 mg each of the longissimus dorsi, triceps brachii, and quadriceps of the Altay sheep to be tested, and store them in liquid nitrogen; extract RNA and store it at -80°C; reverse transcribe it into cDNA, and use the internal reference gene ACTB for PCR amplification increase.

[0040] Prepare 1.5% agarose gel: Dissolve 1.5g agarose in 100ml 0.5×TBE, heat in a microwave oven until fully melted, pour it into a gel tank with a comb inserted after cooling down to room temperature, cool and solidify before use.

[0041] Use the prepared 1.5% agarose gel for electrophoresis detection, voltage 100V, current 50mA, electrophoresis for 35 minutes; after staining with ammonium bromide for 15 minutes, irradiate under the ultraviolet lamp of the gel imager to observe the size and ...

Embodiment 3

[0056] Based on Example 2, the kits containing the SEC61A1 gene provided in the above examples for improving the meat production performance of Altay sheep were applied to mice, small-tailed Han sheep, Karakul sheep, Hotan sheep, Kazak sheep, and Bayinbulak sheep. In the study, the relationship between the expression level of SEC61A1 gene in the longissimus dorsi muscle and the intramuscular fatty acid content, water content and shear force of the longissimus dorsi was observed. See Table 2 for details:

[0057] Table 2: Correlation coefficients between SEC61A1 gene expression and some meat quality indicators

[0058]

fatty acid content

moisture content

longissimus dorsi shear force

altay sheep

0.85

0.92

0.98

Small Tail Han Sheep

0.03

0.15

0.08

Karakul sheep

0.12

0.05

0.17

Hetian sheep

0.12

0.08

0.11

Kazakh sheep

0.06

0.13

0.15

Bayinbulak sheep

0.17

0.11

0.02 ...

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Abstract

The invention discloses an SEC61A1 gene-containing detection kit for improving Altay sheep meat production performance. According to the SEC61A1 gene-containing detection kit disclosed by the invention, a specific primer is self and artificially designed according to an mRNA sequence of a sheep SEC61A1 gene, a forward primer is 5'-TATGTGATGACGGGAATGT-3', a reverse primer is 5'-GGTCTCGCAGATGTTAGTG-3', sample genome DNA is utilized as a template to perform PCR proliferation, fluorescence quantitative PCR detection is performed on a PCR proliferation product, correlation analysis between gene expression quantity and intramuscular fatty acid content, moisture content and meat weight is performed. The SEC61A1 gene-containing detection kit disclosed by the invention not only fills the blank of the research field at home and abroad, but also establishes basis for protecting Altay sheep good stock resources and promoting reasonable development of the stock resources and meanwhile has importantsignificance and actual application valve in quickening Sinkiang mutton sheep industry development and improving high-quality mutton production level.

Description

technical field [0001] The invention relates to an identification method for animal genetic selection and breeding. Specifically, the invention relates to the technical field of a kit for meat production performance selection of Altay sheep. Background technique [0002] Altay sheep is an excellent local mutton sheep breed in Xinjiang. It is a branch of the ancient Kazakh sheep breed. It was raised by the ancestors of Kazakhs (Wusun people) as early as around BC. Lamb has outstanding precocious characteristics, suitable for mutton production, and belongs to the variety of meat and fat. The main production areas of Altay sheep are concentrated in Fuhai County, Fuyun County, Qinghe County and other places in the Altay region in northern Xinjiang, distributed in Altay, Burqin, Jimunai and Habahe counties, but with the introduction and inter-field and inter-interval exchanges , Altay is now basically distributed throughout Xinjiang. Altay sheep are big and strong. Chest broad...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/158C12Q2600/166
Inventor 刘武军赵国艺方超马海玉方丽君刘玲玲于茜褚洪忠
Owner XINJIANG AGRI UNIV
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