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Multi-spectral filter profiling and quality control for flow cytometry

A flow cytometry, optical filter technology, applied in the field of multispectral filter analysis and quality control for flow cytometry, can solve problems such as insufficient quantification and complex flow cytometry measurements

Active Publication Date: 2018-05-04
BIO RAD LAB INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Another problem that further complicates flow cytometry measurements is that the fluorescence that is ultimately delivered to the detector system (which can measure the intensity of this fluorescence) can be extremely weak—so weak that many photodetector systems will cannot adequately quantify its

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  • Multi-spectral filter profiling and quality control for flow cytometry
  • Multi-spectral filter profiling and quality control for flow cytometry
  • Multi-spectral filter profiling and quality control for flow cytometry

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Embodiment Construction

[0042] figure 1 is a depiction of an embodiment of a flow cytometer according to the present invention. As shown, flow cytometer 100 is a self-contained unit that can easily fit on a tabletop. Such as figure 1 As illustrated in , the flow cytometer 100 has a load / unload button 104 to extend and retract the sample tray 102 , which allows insertion of a sample to be tested by the flow cytometer 100 .

[0043] figure 2 yes figure 1 A schematic block diagram of an exemplary optical layout of the detector portion of the embodiment of the flow cytometer illustrated in . Such as figure 2 As shown in , optical layout 200 includes cuvette 204 , objective optics 208 , LED board 210 , series of optical fibers 220 and spectral splitters 222 , 224 , 226 , 228 , 230 . The cuvette 204 has a structure that causes the cells or particles to be interrogated (in this application the term "particle" should be understood to refer to particles, cells or other microscopic items) to flow th...

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Abstract

Disclosed is a system and method for characterizing optical filters in a flow cytometer and optionally checking the operation of detectors in the flow cytometer. In some embodiments, the system may utilize an LED board having an opening through which the fluorescence and side scatter beams, rays, or images pass and light emitting diodes around the opening that emit light having different spectralprofiles. The different spectral profiles allow the system to identify the optical filters that are placed in the flow cytometer, to verify detector operation, to assist in instrumentation troubleshooting, and to provide a quantitative reference for detector comparison.

Description

[0001] Related Application Cross Reference [0002] This application is filed on August 12, 2015 under 35 U.S.C. §119(e) and is titled "MULTI-SPECTRAL FILTER PROFILING ANDQUALITY CONTROL FOR FLOW CYTOMETRY)" and U.S. Provisional Patent Application No. 62 / 204,001 filed on August 11, 2016 and entitled "MULTI-SPECTRAL FILTER PROFILING AND QUALITY CONTROL FOR FLOW CYTOMETRY), which is hereby incorporated by reference in its entirety. Background technique [0003] Flow cytometry has been widely used to analyze cells and various particles. As a diagnostic tool, flow cytometry has been very effective in providing detailed information about the physiology of cells and particles. Flow cytometry has been used in both research laboratories and clinics to analyze cells and other particles. Flow cytometers include conventional hydrodynamically focused sample flow in cuvettes, hydrodynamically focused sample flow with in-air jet sorting flow cytometers, and microfluidic systems that do ...

Claims

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Application Information

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IPC IPC(8): G01N21/93G01J3/28G01J3/32G01J3/36G01J3/40G01N21/17G01N21/25
CPCG01N2015/1006G01N15/1459G01J3/10G01J3/36G01N21/4785G01J3/0218G01J3/027G01J2003/104G01N15/1012G01N15/1434G01N15/01
Inventor 丹尼尔·纳尔逊·福克斯苏珊·亨特
Owner BIO RAD LAB INC