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Method for extracting lycopene

A technology of lycopene and extraction liquid, applied in chemical instruments and methods, hydrocarbon purification/separation, organic chemistry, etc., can solve the problems of high cost, difficult processing process, high volume and tightness requirements, etc.

Active Publication Date: 2021-04-09
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent uses wall-breaking technology and dehydrating agent, so it is more difficult in the subsequent solid-liquid separation step, and a variety of solvents are used, which makes it difficult to recover and recycle the solvent, the process is difficult, and the cost is high
On the other hand, a single batch of solvent extraction requires high equipment volume and tightness, so the cost is high
And because the bacteria are easy to agglomerate, the difficulty of solvent penetration increases, and the extraction yield is also relatively low

Method used

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  • Method for extracting lycopene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019](1) The commercially available trisosporium is fermented, and a fermentationery containing β-carotene is obtained, and the fermentation liquid is filtered to give a wet bacterium. The wet bacteria is boiling to dryness to obtain a triflee, and the water content of the triflee is 7.5%, which is detected, and the content of beta-carotene in the trifaccle is 5.4%.

[0020](2) Take 500 grams of the hemmetroids, and uniformly filled the 200 grams of dried bacteria to 4 tandem 1, 2, 3, 4, each reactive column is provided with sampling. The ports 11, 21, 31, 41 and the pressure gauges 12, 22, 32, 42, and the tandem reaction column is placed in a thermostat of 55 ° C.

[0021](3) Transfer ethyl acetate in the storage tank 3 to the series columns 1, 2, 3, 4. It was detected that the solubility of β-carotenoids at 55 ° C is 2 g / L. The flow rate of the solvent was adjusted to 2 ml / s, and the pressure of the string 1, 2, 3, 4 by the pressure gauges 12, 22, 3, 42 was 0.16 MPa, and sampling w...

Embodiment 2

[0024](1) The commercially available trisosporium is fermented, and a fermentationery containing β-carotene is obtained, and the fermentation liquid is filtered to give a wet bacterium. The wet bacterium is boiling to dryness to obtain a stericide, and the water content of the triflee is 5%. It was detected that the content of beta-carotene in the trifacterium was 8%. The triators were pulverized so that at least 80% of the total amount of the total amount of trifacca was 40% sieve.

[0025](2) Take 1000 g of the experiment, and 400 g of a triamatic body is uniformly filled into four series of reaction columns 1, 2, 3, 4, and each reactive column is provided with a sampling port 11, 21. The 31, 41 and the pressure tables 12, 22, 32, 42, the tandem reaction column is integrally placed in a thermostat of 65 ° C.

[0026](3) Divided n-hexane in the storage tank 3 to the series column 1, 2, 3, and 4. It is detected that the solubility of β-carotene in n-hexane in 60 ° C is 3 g / L. The flow r...

Embodiment 3

[0029](1) Fermentation of the commercially available trisosporium, obtaining a fermentationery containing beta-carotene, and the fermentation liquid is passed to obtain a wet bacterium. The wet bacteria is boiling to dryness to obtain a stericide, and the water content of the triflee is 4%. It was detected that at least 80% of the total amount of the tapered body can be 40 mesh sieve, and the content of β-carotene in the trifacterium is 4%.

[0030](2) Take 10 kg of the trial experiment, and uniformly fill the 4 kg of triflee is equal to 4 series of reaction columns 1, 2, 3, 4, and each reactive column is provided with a sampling port 11. 21, 31, 41 and pressure Tables 12, 22, 32, 42, the tandem reaction column is placed in a 60 ° C incubator.

[0031](3) Dutyry of a acetate of 60 ° C in the storage tank 3 to the series column 1, 2, 3, 4. It was detected that the solubility of β-carotenoid at 60 ° C is 2.5 g / L. The flow rate of adjusting the solvent is 5 ml / s, and the pressure of the ...

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Abstract

The method for extracting lycopene comprises the following steps: (1) fermenting microbial cells containing lycopene to obtain a fermented liquid containing lycopene, and treating the fermented liquid to obtain dried bacteria with a water content less than 10% (2) uniformly filling the dried cells into the reaction columns in series; (3) adding an organic solvent to the reaction columns in series, making the organic solvent pass through the reaction columns in series in turn, extracting lycopene in the bacteria; (4) collecting the lycopene extract, precipitating, crystallizing, filtering, and vacuum drying to obtain lycopene. The invention replaces the traditional batch extraction with continuous extraction, which can effectively improve the extraction yield of lycopene. Further, real-time monitoring of the reaction column during the extraction process can not only ensure sufficient extraction of the bacteria, but also avoid invalid extraction after the extraction of lycopene in the bacteria is completed.

Description

Technical field[0001]The present invention relates to a method of extracting lycopene, in particular a method of extracting lycopene from the product after fermentation of microbacterial.Background technique[0002]Carotenoids are the most popular types of pigments in nature, which can be found in fruit, vegetables, leaves, microorganisms and marine organisms. Some specific examples of carotenoid compounds are: β-carotene, lycopene, lutein, shrimp, corn yellow, hobbin, leukin, etc.[0003]Carotenoids, such as β-carotene, lycopene, and lutein have a variety of important physiological effects such as immunization and antioxidation. At present, β-carotene, lycopene, shrimp, and lutein have been widely used in food, cosmetics, drugs, health products and other industries. Among them, β-carotene is a widely existing fat-soluble carotene, closely related to the health of the human body, not only has a high anti-cancer, antioxidant, anti-radiation, etc., but also in the human body and the anima...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C11/21C07C7/00
CPCC07C7/005C07C11/21
Inventor 汪志明李翔宇陆姝欢杨刚周强
Owner CABIO BIOTECH WUHAN CO LTD