Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of using PLCD4 gene as gastric adenocarcinoma transfer diagnosis marker

A gastric adenocarcinoma and gene technology, applied in the biological field, can solve problems such as unclear molecular mechanism

Active Publication Date: 2018-05-18
GUIZHOU PROVINCIAL PEOPLES HOSPITAL
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for advanced gastric cancer, the underlying molecular mechanism of invasion and metastasis is still unclear.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of using PLCD4 gene as gastric adenocarcinoma transfer diagnosis marker
  • Application of using PLCD4 gene as gastric adenocarcinoma transfer diagnosis marker
  • Application of using PLCD4 gene as gastric adenocarcinoma transfer diagnosis marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Differential expression of PLCD4 gene

[0064] 1. Sample acquisition: 8 cases of gastric adenocarcinoma tissue (including 4 samples with metastasis and 4 samples without metastasis). The above samples are surgical resection specimens of patients with gastric adenocarcinoma. All the above specimens were obtained with the consent of the organizational ethics committee. The clinical data of the tissue samples include: gender, age, tumor size, pathological grade (Edmonson), metastasis or recurrence, etc. The above samples were stored in liquid nitrogen.

[0065] 2. Acquisition of RNA from gastric adenocarcinoma metastasis tissue

[0066] Tissue RNA was extracted using Norgen RNA extraction kit.

[0067] Tissue Lysis:

[0068] 1) In a clean area with less RNase interference, use a mortar containing an appropriate amount of liquid nitrogen to weigh about 20 mg of the isolated tissue sample, and grind it to powder with a pestle;

[0069] 2) Transfer the sample t...

Embodiment 2

[0102] Example 2 Large sample verification of differentially expressed genes

[0103] 1. Collect 80 gastric adenocarcinoma tissues (including 40 samples with metastasis and 40 samples without metastasis) according to the method of Example 1. The above samples are surgical resection specimens of patients with gastric adenocarcinoma. All the above specimens were obtained with the consent of the organizational ethics committee. The clinical data of the tissue samples include: gender, age, tumor size, pathological grade (Edmonson), metastasis or recurrence, etc.

[0104] 2. Tissue total RNA extraction

[0105] Step is with embodiment 1.

[0106] 3. Reverse transcription

[0107] The reverse transcription of RNA was carried out using the reverse transcription kit of TAKARA company.

[0108] 4. QPCR

[0109] (1) Primer design

[0110] QPCR amplification primers were designed according to the coding sequences of PLCD4 gene and GAPDH gene in Genbank, and synthesized by Shanghai ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of using PLCD4 gene as a gastric adenocarcinoma transfer diagnosis marker. A gene chip is applied to screen a differential expression gene related to the gastricadenocarcinoma transfer, and QPCR verification is performed in a big sample; the experimental result shows that the PLCD4 can be used for judging if the gastric adenocarcinoma is transferred or the gastric adenocarcinoma transfer risk is judged in advance. The research result provides a new diagnosis method for clinical diagnosis of gastric adenocarcinoma transfer on a molecular level while provides a new drug target for the gene treatment of the gastric adenocarcinoma transfer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of PLCD4 gene in the diagnosis and treatment of gastric adenocarcinoma metastasis. Background technique [0002] Gastric cancer is a malignant tumor derived from gastric mucosal epithelial cells, mainly adenocarcinoma. In 2008, nearly 1 million cases of gastric cancer were newly diagnosed in the world, and the number of deaths was 740,000. It is the fourth most common cancer in the world and the second most lethal. Although the overall global incidence of gastric cancer has declined, nearly two-thirds of gastric cancer cases are concentrated in economically underdeveloped countries and regions, with high incidence in China and other East Asian countries. According to the latest version of "China Cancer Map" released by "Life Times" in 2014. There are about 3,120,000 new cancer cases in my country every year, with an average of 8,550 cases per day, 6 people are diagn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/178
Inventor 梁丽李岚张玲玲
Owner GUIZHOU PROVINCIAL PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com