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A kind of method for quickly growing seedlings of shequila

A technology of Hequila and seeds, which is applied in the field of regeneration of orchid germplasm resources, can solve the problems of high cost, few wild resources, and harsh environmental conditions, and achieve the goal of reducing a lot of costs, making the seedlings strong and straight, and increasing the germination rate Effect

Active Publication Date: 2019-08-13
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for rapid seedling growth of Hequila, which solves the production problems of long cultivation period and high cost of Orchidaceae plants, and simultaneously solves the difficulty in the source of some precious orchid resources in the prior art. The 1,450-meter-high mountain forest on the trunk or on the rocks in the valley has harsh environmental conditions, few wild resources, good ornamental effect, weak natural reproduction and diffusion ability, difficult natural regeneration, and scarcity of high-quality seedlings. The second-class protected plants have high horticultural ornamental value and are of great significance for the decoration of orchid plants and orchid parks.

Method used

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  • A kind of method for quickly growing seedlings of shequila
  • A kind of method for quickly growing seedlings of shequila

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Material selection and disinfection: 90% of the mature pods in the year of picking are rinsed with detergent, and then rinsed in running water for 20 minutes; seed disinfection treatment: wash the pods, soak them in saturated bleach supernatant for 15 minutes, and wash them with soft wool Scrub the outside of pods. After rinsing, drip rinse with tap water for 0.5 hours, rinse with double-distilled water for 3 times, put them in an ultra-clean workbench and disinfect with 75% alcohol for 30 seconds, pour off the alcohol, add 0.1% mercuric chloride for 6 minutes, and Pour the mercury solution into the waste mercury bottle, rinse it with sterile water 4 times, dry the surface moisture with sterile filter paper, and set aside;

[0021] 2) Bud induction culture: Take the plump and sterilized pods, hold the pods with gun-shaped tweezers, cut the pods vertically with a scalpel, take out the seeds in the pods, and inoculate them in the high-temperature and high-pressure steri...

Embodiment 2

[0030] 1) Material selection and disinfection: 90% of the mature pods in the year of picking are rinsed with detergent, and then rinsed in running water for 20 minutes; seed disinfection treatment: wash the pods, soak them in saturated bleach supernatant for 15 minutes, and wash them with soft wool Scrub the outside of pods. After rinsing, drip rinse with tap water for 0.5 hours, wash with double distilled water twice, put them in an ultra-clean workbench and disinfect with 75% alcohol for 30 seconds, pour off the alcohol, add 0.1% mercuric chloride for 5 minutes, and Pour the mercury solution into the waste mercury bottle, rinse it with sterile water for 3 times, dry the surface moisture with sterile filter paper, and set aside;

[0031] 2) Bud induction culture: Take the plump and sterilized pods, hold the pods with gun-shaped tweezers, cut the pods vertically with a scalpel, take out the seeds in the pods, and inoculate them in the high-temperature and high-pressure steriliz...

Embodiment 3

[0040] 1) Material selection and disinfection: 90% of the mature pods in the year of picking are rinsed with detergent, and then rinsed in running water for 20 minutes; seed disinfection treatment: wash the pods, soak them in saturated bleach supernatant for 15 minutes, and wash them with soft wool Scrub the outside of the pods. After rinsing, drip rinse with tap water for 0.5 hours, rinse with double distilled water for 3 times, put them in an ultra-clean workbench and disinfect with 75% alcohol for 30 seconds, then pour off the alcohol, add 0.1% mercury chloride for 8 minutes, and put Pour the mercury solution into the waste mercury bottle, rinse it with sterile water 4 times, dry the surface moisture with sterile filter paper, and set aside;

[0041] 2) Bud induction culture: Take the plump and sterilized pods, hold the pods with gun-shaped tweezers, cut the pods vertically with a scalpel, take out the seeds in the pods, and inoculate them in the high-temperature and high-pr...

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Abstract

The invention provides a diploprora championii fast seedling forming method, and belongs to the field of arethusa germplasm resources regeneration. According to the method, diploprora championii capsules are collected; under aseptic conditions, bud induction and proliferation rooting are performed; meanwhile, induced culture is completed; transplantable diploprora championii young seedlings are obtained. The arethusa two-step seedling forming method solves the production problems of long culture period, high cost and the like, and simultaneously solves the problems that in the prior art, sources of some precious arethusa resources are difficult to obtain; the plants grow on tree trunks of mountainous region forests with the altitude being 250 to 1450 meters or on ravine rock, and have theharsh requirements on the environmental conditions; the wild resources are few; the ornamental effect is good; the natural breeding capability and spreading diffusion capability are low; the natural updating is difficult; the excellent seed seedlings are scarce and the like. The diploprora championii belongs to national grade 2 protection plants, has higher garden ornamental values, and has the important significance on arethusa garden decoration and orchid park decoration.

Description

technical field [0001] The invention belongs to the field of regeneration of orchid plant germplasm resources, and in particular relates to a method for rapid seedling growth of hedquila. Background technique [0002] Shequila (scientific name: Diploprora championii (Lindl.) Hook. f.): The stem is hard, cylindrical or slightly flattened, often drooping. Leaves papery, sickle-lanceolate or obliquely oblong, base with persistent sheaths, margins sometimes wavy. Racemes opposite to leaves, longer or shorter than leaves, drooping, with 2-5 flowers; flowers fragrant, slightly fleshy, spreading, sepals and petals light yellow; petals smaller than sepals; labial petals white with rose color , the depression below the middle is boat-shaped, without distance, about 10 mm long, about 4 mm wide, slightly 3-lobed. Capsule cylindrical. The flowering period is from February to August, and the fruiting period is from March to September. [0003] Born on tree trunks or rocks in valleys ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈世品肖春梅何碧珠刘江枫马良林文俊
Owner FUJIAN AGRI & FORESTRY UNIV
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