A kind of method for quickly growing seedlings of shequila
A technology of Hequila and seeds, which is applied in the field of regeneration of orchid germplasm resources, can solve the problems of high cost, few wild resources, and harsh environmental conditions, and achieve the goal of reducing a lot of costs, making the seedlings strong and straight, and increasing the germination rate Effect
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Embodiment 1
[0020] 1) Material selection and disinfection: 90% of the mature pods in the year of picking are rinsed with detergent, and then rinsed in running water for 20 minutes; seed disinfection treatment: wash the pods, soak them in saturated bleach supernatant for 15 minutes, and wash them with soft wool Scrub the outside of pods. After rinsing, drip rinse with tap water for 0.5 hours, rinse with double-distilled water for 3 times, put them in an ultra-clean workbench and disinfect with 75% alcohol for 30 seconds, pour off the alcohol, add 0.1% mercuric chloride for 6 minutes, and Pour the mercury solution into the waste mercury bottle, rinse it with sterile water 4 times, dry the surface moisture with sterile filter paper, and set aside;
[0021] 2) Bud induction culture: Take the plump and sterilized pods, hold the pods with gun-shaped tweezers, cut the pods vertically with a scalpel, take out the seeds in the pods, and inoculate them in the high-temperature and high-pressure steri...
Embodiment 2
[0030] 1) Material selection and disinfection: 90% of the mature pods in the year of picking are rinsed with detergent, and then rinsed in running water for 20 minutes; seed disinfection treatment: wash the pods, soak them in saturated bleach supernatant for 15 minutes, and wash them with soft wool Scrub the outside of pods. After rinsing, drip rinse with tap water for 0.5 hours, wash with double distilled water twice, put them in an ultra-clean workbench and disinfect with 75% alcohol for 30 seconds, pour off the alcohol, add 0.1% mercuric chloride for 5 minutes, and Pour the mercury solution into the waste mercury bottle, rinse it with sterile water for 3 times, dry the surface moisture with sterile filter paper, and set aside;
[0031] 2) Bud induction culture: Take the plump and sterilized pods, hold the pods with gun-shaped tweezers, cut the pods vertically with a scalpel, take out the seeds in the pods, and inoculate them in the high-temperature and high-pressure steriliz...
Embodiment 3
[0040] 1) Material selection and disinfection: 90% of the mature pods in the year of picking are rinsed with detergent, and then rinsed in running water for 20 minutes; seed disinfection treatment: wash the pods, soak them in saturated bleach supernatant for 15 minutes, and wash them with soft wool Scrub the outside of the pods. After rinsing, drip rinse with tap water for 0.5 hours, rinse with double distilled water for 3 times, put them in an ultra-clean workbench and disinfect with 75% alcohol for 30 seconds, then pour off the alcohol, add 0.1% mercury chloride for 8 minutes, and put Pour the mercury solution into the waste mercury bottle, rinse it with sterile water 4 times, dry the surface moisture with sterile filter paper, and set aside;
[0041] 2) Bud induction culture: Take the plump and sterilized pods, hold the pods with gun-shaped tweezers, cut the pods vertically with a scalpel, take out the seeds in the pods, and inoculate them in the high-temperature and high-pr...
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