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Method for using four sheath blight resistance genes of Yangdao 4

A resistance gene, sheath blight technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of reduced and few sheath blight resistance

Inactive Publication Date: 2018-05-22
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rice research community has identified many QTLs related to rice sheath blight resistance, but which QTLs jointly regulate rice sheath blight resistance? Which QTLs can be used simultaneously in a breeding process? We don't know much about these issues
According to the molecular markers linked to rice sheath blight resistance genes that have been reported so far, it is often impossible to aggregate several different resistance genes together in order to obtain rice materials with enhanced resistance. Yes, because the randomly selected resistance genes are likely to have epistasis interaction effects between genes, resulting in reduced sheath blight resistance of materials aggregated with multiple resistance genes

Method used

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  • Method for using four sheath blight resistance genes of Yangdao 4
  • Method for using four sheath blight resistance genes of Yangdao 4
  • Method for using four sheath blight resistance genes of Yangdao 4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Example 1: Application of D715, D804, D948, and RM409 markers in the segregating population of the F6 generation of the hybrid combination of rice variety Lemont and rice variety Yangdao No. 4.

[0009] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F6 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F6 segregation population including 219 lines was sown in May 2013 at the China Rice Research Institute Experimental Field in Fuyang District, Hangzhou.

[0010] 2. Using markers D715, D804, D948, and RM409 to perform PCR detection on each strain in the recombinant inbred strain segregation population of the 219 strains, respectively. The band pattern of D715 ma...

Embodiment 2

[0015] Example 2: Application of D715, D804, D948, and RM409 markers in the segregating population of the F9 generation of the hybrid combination of rice variety Lemont and rice variety Yangdao No. 4.

[0016] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F9 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F9 segregation population including 219 lines was sown in May 2015 at the China Rice Research Institute Experimental Field in Fuyang District, Hangzhou.

[0017] 2. Using markers D715, D804, D948, and RM409 to perform PCR detection on each strain in the recombinant inbred strain segregation population of the 219 strains, respectively. The band pattern of D715 ma...

Embodiment 3

[0022] Example 3: Application of D715, D804, D948, and RM409 markers in the segregating population of the F13 generation of the hybrid combination of rice variety Lemont and rice variety Yangdao 4.

[0023] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F13 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F13 segregation population including 219 lines was sown in May 2017 at the China Rice Research Institute Experimental Field in Fuyang District, Hangzhou.

[0024] 2. Using markers D715, D804, D948, and RM409 to perform PCR detection on each strain in the recombinant inbred strain segregation population of the 219 strains, respectively. The band pattern of D715 mar...

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Abstract

The invention discloses a method for using four sheath blight resistance genes of Yangdao 4. According to the method, PCR amplification is performed on different strains in a recombinant inbred segregation line produced by Lemont and Yangdao 4 by using four markers D715, D804, D948 and RM409 separately, wherein a band type of Yangdao 4 amplified by the marker D715 is named as A, and a band type ofLemont amplified by the marker D715 is named as E; a band type of Yangdao 4 amplified by the marker D804 is named as B, and a band type of Lemont amplified by the marker D804 is named as F; a band type of Yangdao 4 amplified by the marker D948 is named as C, and a band type of Lemont amplified by the marker D948 is named as G; and a band type of Yangdao 4 amplified by the marker RM409 is named asD, and a band type of Lemont amplified by the marker RM409 is named as H. Strains with more band types of A, B, C and D better resist sheath blight, and strains with more band types of E, F, G and Hbetter sense sheath blight. The method uses four rice sheath blight-resistant genes at the same time so as to breed the sheath blight resistant rice, thereby greatly improving the breeding efficiency.

Description

technical field [0001] The invention relates to a method for utilizing four sheath blight resistance genes of Yangdao No. 4. The method can use four molecular markers to simultaneously polymerize four sheath blight resistance genes to select rice materials resistant to sheath blight . Background technique [0002] Rice is an important food crop related to my country's food security. The traditional methods of breeding sheath blight-resistant rice in my country's rice breeding community are all through phenotypic selection, that is, inoculating and identifying rice plants with the pathogenic bacteria of sheath blight at different rice developmental stages. Because most of the traditional inoculation identification is carried out under field planting conditions, the results of inoculation identification have large errors and poor accuracy, and the inoculation results of different years are different, resulting in low accuracy of inoculation identification results. The select...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13
Inventor 曾宇翔杨长登季芝娟梁燕
Owner CHINA NAT RICE RES INST
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