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Method for separating and extracting bomeol glycoside from Ophiopogon japonicus

A technology of borneolside and Ophiopogon japonicus is applied in the field of separation and purification of traditional Chinese medicines, which can solve the problems of successful extraction or synthesis without a reference substance of borneolside, and a way to purchase the reference substance without borneolside, etc., and achieves short cycle, low cost, Simple steps to operate

Inactive Publication Date: 2018-05-29
CHIATAI QINGCHUNBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no way to purchase the reference substance of borneol glycoside in the market, and there is no related literature about the successful extraction or synthesis of the reference substance of borneol glycoside

Method used

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  • Method for separating and extracting bomeol glycoside from Ophiopogon japonicus
  • Method for separating and extracting bomeol glycoside from Ophiopogon japonicus
  • Method for separating and extracting bomeol glycoside from Ophiopogon japonicus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 4 parts of 2 kg of Ophiopogon japonicus roots (underground part, collected according to the 2015 edition of the Chinese Pharmacopoeia) (the content of borneoside in the medicinal material is 0.013%, that is, 0.26g of borneoside), and extract them in the following ways, and calculate Extraction rate of borneoside:

[0026] ① Add 2kg of Ophiopogon japonicus roots, add 4L of 85% ethanol aqueous solution to reflux and extract 3 times, each time for 1 hour, keep slightly boiling, filter the medicinal residues, combine the extracts, detect by HPLC-ELSD method, the quality extraction rate is 89.3 %.

[0027] ② Add 2kg of Ophiopogon japonicus roots, add 8L of 85% ethanol aqueous solution to reflux and extract twice, each time for 2 hours, keep slightly boiling, filter the medicinal residues, combine the extracts, detect by HPLC-ELSD method, the quality extraction rate is 91.2 %.

[0028] ③ 2 kg of Ophiopogon japonicus roots, add 8 L of ethanol aqueous solution with volum...

Embodiment 2

[0032] Get 20kg of root tubers of Ophiopogon japonicus (medicinal material borneoside detection content is 0.014%, namely containing borneoside 2.8g), add 60L volume concentration 90% ethanol aqueous solution reflux extraction 2 times, each time 2 hours, keep slight boiling, Filter the medicinal residues and combine the extracts; after concentrating to alcohol-free (about 6L), add deionized water to 80L, mix well and then refrigerate at 2-10°C for 36 hours to precipitate and remove impurities, and take the supernatant and filter it through a plate containing activated carbon Obtain 75L of water-sinking liquid, then go to D101 macroporous resin (8L) to separate, use 80L deionized water and 25L volume concentration 20% ethanol aqueous solution, 25L volume concentration 30% ethanol aqueous solution, 30L volume concentration 40% ethanol aqueous solution as washing Deliquidation and segmental elution, in which ethanol eluents with different concentrations need 5L as a receiving port...

Embodiment 3

[0034]Get 200kg of root tubers of Ophiopogon japonicus (medicinal material borneoside detection content is 0.016%, promptly contain borneoside 32.0g), add 600L volume concentration 90% ethanol aqueous solution reflux extraction 3 times, the first, the second time each time 2 hour, the third time for 1 hour, keep slightly boiling, filter the dregs, and combine the extract; after concentrating until alcohol-free, add deionized water to 500L, mix well, and then refrigerate at 2-10°C for 24h to precipitate and remove impurities, take the above The clear liquid was filtered through a plate frame containing activated carbon to obtain 480L of water-sinking liquid, and then separated by D1400 macroporous resin (80L), followed by 800L deionized water and 250L volume concentration of 20% ethanol aqueous solution, 250L volume concentration of 30% ethanol aqueous solution, and 300L volume concentration Concentration of 40% ethanol aqueous solution is used as the eluent for segmental elutio...

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Abstract

The invention discloses a method for separating and extracting bomeol glycoside from Ophiopogon japonicus. The method comprises carrying out full heating reflux extraction on the root tuber of Ophiopogon japonicus through an ethanol aqueous solution with the volume concentration of 85%-95% as an extraction solution to obtain extract, and separating to purify the extract to obtain bomeol glycoside.The method has simple processes, realizes a low cost, has a short period, realizes bomeol glycoside purity of 98% or more and can produce bomeol glycoside in the gram level.

Description

(1) Technical field [0001] The invention relates to a method for extracting and separating characteristic components of a traditional Chinese medicine, in particular to a method for extracting and separating borneoside, a characteristic component of Ophiopogon japonicus, belonging to the technical field of separation and purification of Ophiopogon japonicus. (2) Background technology [0002] Ophiopogon japonicus is an evergreen herb belonging to the genus Liliaceae. Distributed in my country's Sichuan, Hubei, Fujian, Guizhou, Zhejiang and other provinces. Since ancient times, people have used the underground tubers of Ophiopogon japonicus as medicinal materials, which are now included in the Chinese Pharmacopoeia, and are mainly used to treat diseases such as dryness of the lungs, dry cough, and thirsty wounds. Modern studies have shown that Ophiopogon japonicus roots contain a large amount of flavonoids, saponins, polysaccharides, polyphenols, volatile oils, borneosides a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/26C07H1/08
CPCC07H1/08C07H15/26
Inventor 凌益平李静慧张善飞
Owner CHIATAI QINGCHUNBAO PHARMA
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