Bacterium inhibition level identification method based on plate experiment and deep learning

Abstract

The invention discloses a bacterium inhibition level identification method based on a plate experiment and deep learning. The method comprises the following steps of: S1, determining the position of amedicine sensitive paper sheet; S2, measuring the size of an inhibition zone; S3, constructing a sample set: constructing a sample set having a label through using a deep learning model, wherein thesample set can be used for training a model; S4, constructing and training a model: constructing a model that can be used for identifying the medicine sensitive paper sheet, and training the model byusing the sample set; S5, adopting the model to identify the medicine sensitive paper sheet; and S6, judging the bacterium inhibition level of a medicine: identifying the type of the medicine by usingmodel, inquiring a corresponding bacterium inhibition standard from a database, determining an interval to which the diameter of the inhibition zone belongs in the standard, and obtaining the bacterium inhibition level of the medicine. With the method of the invention adopted, an operator only needs to photograph a plate image so that the operator can identify the medicine type to which the medicine sensitive paper sheet belongs and the size of the inhibition zone corresponding to the medicine type from the image, and therefore, the automatic identification of bacterium inhibition levels canbe possible.

Description

technical field [0001] The present invention relates to the technical field of bacteriostasis identification, in particular to a bacteriostasis identification method based on plate experiments and deep learning. Background technique [0002] A petri dish, also called a petri dish, is a laboratory vessel used for microorganism or cell culture, consisting of a flat disc-shaped bottom and a cover, generally made of glass or plastic. The materials of petri dishes are basically divided into two categories, mainly plastic and glass. Glass can be used for plant materials, microbial culture and animal cell adherent culture. The plastic ones may be made of polyethylene materials, and there are disposable and multiple-use ones, which are suitable for laboratory inoculation, streaking, and isolation of bacteria, and can be used for the cultivation of plant materials. [0003] Drug susceptibility test is referred to as drug susceptibility test (or drug resistance test). A microbiologi...

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Problems solved by technology

These operations are cumbersome manual operations, occupying the working time of the operator and consuming a lot of energy

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