Frog heart perfusion experimental device and application method thereof
An experimental device and frog heart perfusion technology, which is applied in the fields of biological experimental devices and medical functions, can solve the problems of calculation result error and insufficient calculation result accuracy, and achieve the effect of improving accuracy and ensuring rigor and accuracy.
Inactive Publication Date: 2018-05-29
梁辰
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Problems solved by technology
On the one hand, because the number of droplets corresponding to 1mL volume of liquid is not accurate enough, the accuracy of the calculation results is not enough; Adding the experimental substance, and the difference in the liquid composition causes the volume of the droplet to change. During the experiment, the previously established conversion standard (the number of droplets corresponding to 1mL volume of liquid) was used to calculate the cardiac output, resulting in obvious errors in the calculation results.
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[0030] Such as figure 1 and figure 2A frog heart perfusion experimental device shown includes an arterial cannula 3 and a venous cannula 19 that communicate with the ventricle 1 and the atrium 2 respectively, and the other end of the arterial cannula 3 is connected to a branch of the three-way connecting pipe 4 , the upward branch of the three-way connecting pipe 4 is connected to the lower end of the buffer tube 5, the other branch of the three-way connecting pipe 4 is connected to the arterial tube 6, and the other end of the arterial tube 6 is connected to the dropper 7, and the dripping A venous tube 17 is arranged directly below the tube 7, and the lower end of the venous tube 17 is connected with a venous cannula 19 to form a circulation system; the bottom of the burette 7 is provided with a dripping electrode 8, and the dripping electrode 8 is connected by a wire 9. Biological function experiment system 10; a metering tube 11 is arranged between the described dropper ...
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The invention discloses a frog heart perfusion experimental device and an application method thereof, which belong to the field of medical function and biological experimental devices. The frog heartperfusion experimental device is based on the existing experimental device provided with a drop recording device, a metering tube is arranged just below the drop recording device, the metering tube ismarked with a tag line on a tube wall at a position at which a liquid level of 1 ml volume liquid locates, two sides of the metering tube are provided with an infrared emitter and an infrared receiver, and infrared rays propagated between the infrared emitter and the infrared receiver are positioned on the same plane as the tag line; and the infrared receiver is connected with a biological function experimental system by means of a lead wire, and the biological function experimental system is provided with a display screen. By additionally arranging devices such as the metering tube, a liquiddrop number corresponding to the 1 ml volume liquid under the current experiment condition can be precisely determined, each experiment condition has a corresponding conversion standard, the precision of data is significantly improved, and the preciseness and accuracy of experiments are ensured.
Description
technical field [0001] The invention relates to the fields of medical functional science and biological experiment devices, in particular to a frog heart perfusion experiment device and an application method thereof. Background technique [0002] Isolated heart perfusion is one of the important means of studying heart function, and it is widely used in teaching and scientific research in medicine, biology, and pharmacy. Using the isolated heart perfusion system can visually observe the activity of the heart, and study the effects of cardiac diastolic and systolic function, heart rate and rhythm changes, preload and postload, and various body fluid factors on cardiac function. Because the frog heart has the advantages of easy preparation, no need for constant temperature and oxygenation, which makes the frog heart survive for a long time, it is often used for isolated heart perfusion experiments. [0003] Perfusion of the isolated heart using the frog heart is a more commonl...
Claims
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IPC IPC(8): G09B23/28G09B23/36
CPCG09B23/28G09B23/36
Inventor 梁辰
Owner 梁辰