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Application of Tianqingdibai Extract as Gene Therapy Sensitizer

A technology of azure, gene therapy, applied in gene therapy, medical preparations containing active ingredients, drug combinations, etc.

Inactive Publication Date: 2021-01-15
杨堃
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no related report about Tianqingdibai and its extracts as gene therapy sensitizers

Method used

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  • Application of Tianqingdibai Extract as Gene Therapy Sensitizer
  • Application of Tianqingdibai Extract as Gene Therapy Sensitizer
  • Application of Tianqingdibai Extract as Gene Therapy Sensitizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Dry and pulverize Tianqingdibai, add 10 times by weight of water, heat to 100°C, keep warm for 3 hours, extract twice, filter, discard the filter residue, and combine the filtrates; the filtrate is concentrated to a relative density of 1.1-1.2(50 ℃ test), add 95% ethanol to the concentrated filtrate to make the final concentration of ethanol 60%, let stand overnight, filter, the filtrate is separated by membrane separation method to separate the effective part group with molecular weight between 5000-15000, concentrate After drying, the extract of azure radix is ​​obtained.

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Abstract

The invention discloses applications of herba gnaphalii japonici extract in preparing a gene therapy sensitizer. According to the technical scheme, the functions that the herba gnaphalii japonici extract can up-regulate the expression level of Cx43 and Cx26 of tumor cell connexin and promote the gap junction communication are discovered for the first time, so that the synergistic interaction function with a suicide gene treatment system is achieved; further, the construction of a suicide gene stable cell strain for the human melanoma verifies that the herba gnaphalii japonici extract has the synergistic interaction function for the suicide gene treatment of the human melanoma, and thus the herba gnaphalii japonici extract can be adopted as the sensitizer for gene therapy. Meanwhile, the herba gnaphalii japonici extract intrinsically has a certain anti-tumor effect which is overlapped with the enhanced gene therapy effect, and the anti-tumor effect can be exerted to the maximum.

Description

technical field [0001] The invention relates to the technical field of gene therapy, in particular to the application of an extract of Celestia radix as a gene therapy sensitizer. Background technique [0002] Malignant tumors are currently one of the main causes of human death. With the development of cell biology and molecular biology, gene therapy plays an increasingly important role in the clinical treatment of malignant tumors, especially tumor suicide gene therapy. After the traditional treatment methods (such as surgery, chemotherapy, radiotherapy, etc.), it is the most promising method to challenge the treatment of tumors. [0003] The so-called suicide gene therapy refers to the introduction of suicide genes (genes of certain viruses or bacteria) into target cells, and the sensitivity is mediated by encoding the enzymes of viruses or bacteria. The expressed enzymes can catalyze the conversion of non-toxic prodrugs into cytotoxic substances, which cause the cells th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P35/00A61K36/28
CPCA61K36/28A61K48/00A61K2300/00
Inventor 杨堃
Owner 杨堃
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