γδ-T cell exosomes for tumor immunotherapy and preparation method thereof
An immunotherapy and exosome technology, which is applied in the field of tumor treatment, can solve the problems of limited curative effect and achieve the effects of avoiding diffusion difficulties, inhibiting tumor growth, and enhancing anti-tumor immunity
Active Publication Date: 2020-12-22
SICHUAN CANCER HOSPITAL
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Problems solved by technology
At present, more studies are using DC-derived exosomes to carry tumor-specific antigens for anti-tumor therapy, but phase I clinical trials have shown very limited efficacy
Method used
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Embodiment 1
[0038] A kind of γδT cell exosomes for tumor immunotherapy, the γδT cell exosomes are loaded with miR-138.
[0039] The expression level of miR-138 is 500 copy / ul.
Embodiment 2
[0041] A kind of γδT cell exosomes for tumor immunotherapy, the γδT cell exosomes are loaded with miR-138.
[0042] The expression level of miR-138 is 1000 copy / ul.
Embodiment 3
[0044] A kind of γδT cell exosomes for tumor immunotherapy, the γδT cell exosomes are loaded with miR-138.
[0045] The expression level of miR-138 is 2000 copy / ul.
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The present invention provides a γδT cell exosome for tumor immunotherapy, and the γδT cell exosome is loaded with miR-138. Using γδT cell-derived exosomes (γδTDE) carrying exogenous miR-138 to directly target and kill tumor cells, while inhibiting PD-1 / PD-L1 to enhance the anti-tumor immune activity of T lymphocytes / NK cells, through direct And indirect ways to achieve the effect of inhibiting tumor growth / invasion / metastasis.
Description
technical field [0001] The invention belongs to the technical field of tumor treatment, and in particular relates to a γδT cell exosome used for tumor immunotherapy. Background technique [0002] Tumor immunotherapy has received great attention recently, and was rated as the top ten scientific and technological breakthroughs of the year by Science in 2013, and has broad application prospects. Despite promising results in the treatment of hematological malignancies and malignant melanoma, immunotherapy has had little effect in most solid tumors. [0003] T lymphocytes are the final effector cells of anti-tumor immunity. Adoptive Cell Therapy (ACT), which reinfuses T lymphocytes activated by tumor-associated antigens (TAAs) to treat tumors, has received extensive attention in recent years. . In particular, chimeric antigen receptor (CAR)-modified T cell (CAR-T) technology has achieved rapid development and has shown strong efficacy in the treatment of hematological malignanc...
Claims
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IPC IPC(8): C12N5/10C12N15/867A61K31/7088A61K47/46A61K35/17A61P35/00
CPCA61K31/7088A61K35/17A61K47/46C12N5/0636C12N15/86C12N2510/00C12N2740/15043C12N2800/107A61K2300/00
Inventor 朱桂全曹邦荣
Owner SICHUAN CANCER HOSPITAL