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Circulating miRNA marker related toovarian cancer assisted diagnosis and application of marker

An auxiliary diagnosis and marker technology, applied in the field of genetic engineering and oncology, can solve the problem of incomplete consistency of research results, and achieve the effect of high sensitivity and specificity, minimally invasive acquisition and good stability

Active Publication Date: 2018-06-01
朱伟
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  • Claims
  • Application Information

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Problems solved by technology

However, due to differences in research methods and included populations, the results of the studies were not completely consistent.

Method used

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  • Circulating miRNA marker related toovarian cancer assisted diagnosis and application of marker
  • Circulating miRNA marker related toovarian cancer assisted diagnosis and application of marker
  • Circulating miRNA marker related toovarian cancer assisted diagnosis and application of marker

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Experimental program
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Embodiment Construction

[0046] The inventor collected a large number of venous serum and plasma samples from the First Affiliated Hospital of Nanjing Medical University from 2015 to 2016, and selected 211 cases of ovarian cancer and 216 cases of ovarian cancer by sorting out the sample data. The normal control sample was used as the experimental sample for the initial screening of Exiqon miRNA qPCR panel chip and subsequent series of qRT-PCR verification. At the same time, 22 cases of ovarian cancer tissues and 22 cases of paracancerous tissues were retained. The selected serum samples of patients were all from patients who had not undergone surgery, radiotherapy and chemotherapy intervention and were pathologically confirmed as ovarian cancer. The demographic data and clinical data of these samples were collected systematically.

[0047] Refer to the flowchart ( figure 1 and figure 2 ), 20 cases of ovarian cancer samples and 10 cases of normal controls were randomly selected from the serum and p...

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Abstract

The invention discloses a circulating miRNA marker related to ovarian cancer assisted diagnosis and an application of the marker. The marker includes one or more of the following plasma markers: miR-205-5p, miR-145-5p, miR-10a-5p, miR-346 and miR-328-3p as well as the following serum markers: miR-200c-3p, miR-346, miR-127-3p, miR-143-3p and miR-205-5p. The circulating miRNA, as a novel biologicalmarker, has the characteristics of being good in stability, minimally invasive and easy for acquisition, and high in sensitivity and specificity. The molecular markers, with development and utilization, can offer a direction for diagnosis and further treatment of various diseases including tumors. Through the research, the ovarian cancer circulating miRNA markers, which has a clinical diagnosis potential, can be obtained with high pertinence. Based upon researches, the reliability and the repeatability of the group of miRNA as noninvasive markers for diagnosing ovarian cancer are proved.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and oncology, and relates to a circulating miRNA marker related to the auxiliary diagnosis of ovarian cancer and its application. Background technique [0002] Ovarian cancer (OC) is one of the female malignancies with high morbidity and mortality worldwide. According to Cancer Statistics data in 2017, ovarian cancer is the fifth leading cause of death in women. Since the early clinical manifestations of ovarian cancer patients have no obvious specificity, the early diagnosis rate in our country is low, and most of the ovarian cancer patients are in the advanced stage. Although the combined decompression surgery combined with radiotherapy and chemotherapy, the 5-year survival rate is only about 30%. Moreover, pelvic examination, transvaginal B-ultrasound and tumor markers, such as carbohydrate antigen 125 (CA125), have been widely used clinically, but they still cannot detect diseases early an...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 朱伟陈彦贾雪梅殷茵周鑫王魏魏
Owner 朱伟
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